flow cytometry

FlowWeb, a free, web-based platform for flow cytometry data analysis

FlowWeb provides a web-based platform for flow cytometry analysis, offering automated workflows and visualization tools comparable to commercial software.

Active Surveillance Reveals a Systemic Pro-Resolving Th2 Immune Program Linked to 1 Desmoid Tumor Regression

This study identifies a Th2 immune program linked to desmoid tumor regression through active surveillance and multi-omics analysis.

Limited protection against early-life cytomegalovirus infection results from deficiency of cytotoxic CD8T cells

This study shows that deficiency of cytotoxic CD8 T cells in early life leads to limited protection against cytomegalovirus infection.

Distinct contribution of autoreactive B cell Bruton's tyrosine kinase signaling to neuroinflammation

This study shows that deleting BTK in B cells reduces EAE severity by impairing autoreactive B cell functions, supporting BTK as a therapeutic target in MS.

Arachidonic acid availability controls neutrophil swarm initiation and scaling

This study identifies arachidonic acid (AA) as a key regulator of neutrophil swarming, showing that AA release is necessary and sufficient for swarm initiation and scaling.

Tissue-specific adaptation of human T cells is preserved during tissue inflammation

The paper examines tissue-specific T cell adaptations in healthy and inflamed human mucosal tissues, finding that these adaptations are largely maintained during inflammation.

Derivation of functional early gestation decidual natural killer cell subtypes from induced pluripotent stem cells

This study identifies functional differences in term decidual natural killer cells compared to early gestation and develops a protocol to generate functional early gestation-like dNK from iPSC.

Enhanced human antigen-specific B cell responses using in vitro 3D tonsil cultures containing stromal cells

This study shows that 3D tonsil cultures with stromal cells enhance antigen-specific B cell responses compared to 2D cultures and improve follicle-like structure formation.

Multiple sclerosis-associated HLA demarcates EBV-specific CD8+ T cells with an exhausted and brain residency phenotype

The paper examines how HLA-B7 and HLA-A2 alleles influence EBV-specific CD8+ T cell effector phenotypes and their association with MS, finding HLA-B7-restricted cells are more exhausted and CNS-resident in MS patients.

Serial vaccination expands and refines human CD4+ T cell memory

This study tracks CD4+ T cell memory development after three mRNA vaccinations in SARS-CoV-2-naive individuals, showing primary vaccination drives memory pool size and boosters refine its quality and breadth.

Immunomodulatory Effects of Selenium on T-Cell-Mediated Responses in Thyroid Eye Disease

This study investigates how selenium affects T-cell responses in thyroid eye disease, finding it reduces Tfh and Th1 cells and their associated autoantibodies through ferroptosis and apoptosis.

Title: Multimodal profiling reveals Mycobacterium tuberculosis restricts lung mitochondrial immunometabolism to promote pathogenesis

This study identifies how M. tuberculosis manipulates lung mitochondrial metabolism to promote pathogenesis and reveals a mitochondrial immunometabolic signature associated with protective immunity.

Intra-Tumoral Treg Ablation Unleashes NK Cell-Mediated Control of CD8 T Cell-Resistant Tumors

Intra-tumoral Treg ablation enhances NK cell-mediated tumor control by restoring IL-2 production, effective against both MHC I+ and MHC I- cancers without systemic toxicity.

An imaging flow cytometry method to study platelet-monocyte aggregates using Long COVID as a model

This study uses imaging flow cytometry to quantify platelet-monocyte aggregates in Long COVID patients and healthy controls, finding significantly elevated levels and altered attachment patterns in patients.

Clinical development of gene edited tacrolimus-resistant Treg (FKBP12KO-Treg) to enable simultaneous immunosuppression and support of immune regulation

This paper develops gene-edited tacrolimus-resistant Treg cells (FKBP12KO-Treg) for clinical use in transplant recipients, showing they maintain immunoregulatory function while resisting tacrolimus.

NG2-targeting macrophages inhibit 3D invasion of patient-derived glioblastoma spheroids

This study shows NG2-targeting CAR-Ms engulf glioblastoma cells and block over 85% of their 3D invasion.

Pi16+ fibroblast-derived Csf1 shapes skin topography

The paper investigates the role of Pi16+ fibroblasts in regulating macrophage homeostasis and skin architecture by analyzing the effects of Csf1 deletion in these cells.

Heterogeneous signaling pathways are critical for the persistence of memory T cells in spleen and bone marrow

This study identifies tissue-specific signaling pathways critical for memory T cell persistence in spleen and bone marrow.

Neutrophil Reprogramming Underlie Vasculopathy and Lung Disease in Systemic Sclerosis

This study identifies neutrophil reprogramming in systemic sclerosis (SSc) driven by HMGB1+ extracellular vesicles, leading to lung inflammation and fibrosis, and suggests therapeutic targeting of HMGB1.

Circulating Immune Cells are Associated with Non-Inflammatory Pain in Rheumatoid Arthritis

The paper identifies immune cell populations and gene expression differences associated with non-inflammatory pain in RA patients using flow cytometry and single-cell RNA-seq.

Multi-parameter spectral flow cytometry panel for immune phenotyping of murine B and T cell responses

The paper develops a 31-parameter spectral flow cytometry panel to analyze immune responses in mouse B and T cells across multiple tissues.

CD31+ T-cells express greater VEGF-A and CXCR4 levels than CD31- counterparts with VEGF-A expression exacerbated with advancing age

The paper compares VEGF-A and CXCR4 levels in CD31+ and CD31- T-cells across age groups, finding higher VEGF-A in CD31+ T-cells and increased levels with age.

Overriding bioprocess perturbations with a cell machine interface for reliable microbial stress-response control

This study evaluates the Segregostat system's ability to control stress responses in microbial cells under industrial bioreactor conditions using automated flow cytometry.

Neutrophil Fc gamma RI expression as a determinant of oxidative responses in human blood

This study shows that neutrophil Fc gamma RI expression determines oxidative responses in human blood, with increased ROS production linked to Fc gamma RI levels in both healthy individuals and lupus patients.

Nano-flow cytometry of single extracellular vesicles reveals subpopulation differences across cell types and pharmacological perturbations

This study uses nano-flow cytometry to analyze single extracellular vesicles, revealing subpopulation differences across cell types and drug treatments.

Pattern of Circulating Mesenchymal Stromal Cells and Hematopoietic Progenitor and Stem Cells in the Peripheral Blood of Trauma Patients with and without Hemorrhagic Shock

The paper examines circulating mesenchymal stromal cells and hematopoietic progenitor/stem cells in trauma patients with and without hemorrhagic shock, finding that persistent early elevation of these cells and cytokines predicts worse outcomes.

Adult patients with autoinflammation of unknown origin partially phenocopy the immune presentation of Still's disease

The paper compares immunological profiles of patients with autoinflammation of unknown origin to healthy controls and Still's disease patients, identifying shared biomarkers and immune features.

A double-staining automated flow cytometry method for real-time monitoring of bacteria in continuous bioreactors

This paper introduces an automated flow cytometry method for real-time monitoring of bacterial cultures in bioreactors, using double staining to track DNA content and replication.

MD Biophysics Photobiomodulation Plasma (PPT)/ Very Small Embryonic like (VSEL) Antibody Marker Trend Analysis

This study examines changes in VSEL-associated antibody markers after applying PBM to autologous PRP, finding directional increases in three of four markers despite no statistical significance.

Spontaneous Pregravid Obesity Reshapes Fetal Immune Ontogeny in a Nonhuman Primate Model

This study shows that pregravid obesity in rhesus macaques alters fetal immune development, leading to immune dysregulation and increased disease susceptibility in offspring.