flow cytometry

Beyond ex vivo and in vivo CAR T: antigen-driven CAR T (adCAR-T) expansion method enables rapid, physiological CAR T cells programming.

This paper introduces adCAR-T, an antigen-driven CAR T-cell expansion method that enables rapid, physiological CAR T cell programming without ex vivo manufacturing or lymphodepleting chemotherapy.

Neutrophil subsets in SLE exhibit increased glycolysis that correlates with disease activity

This study identifies increased glycolysis in SLE neutrophil subsets, correlating with disease activity and metabolic flexibility.

Senescent cells induce vascular MHC II to recruit CD4+ T cells and drive inflammation in aging adipose tissue

This study identifies senescent cells as drivers of age-related inflammation in adipose tissue by inducing vascular MHC II and recruiting CD4+ T cells through an IFN-gamma feedback loop.

Rac2 Hyperactivity Drives Neutrophil Degranulation, Myeloperoxidase Deficiency, and Lymphopenia

This study identifies Rac2 hyperactivity as a cause of neutrophil dysfunction and T cell lymphopenia in patients with RAC2E62K mutations.

Characterization of tumor interactions with the immune system in an autochthonous mouse model of glioblastoma

This paper studies immune responses in a mouse model of glioblastoma, finding that large tumors induce immune responses at border sites but also generate immunosuppressive necroses that limit therapeutic benefit.

Development and characterisation of an optimised in vitro differentiation protocol for deriving hepatocyte-like cells from mouse embryonic stem cells

This study develops and evaluates two cytokine-based protocols to generate functional hepatocyte-like cells from mouse embryonic stem cells, with the advanced cocktail producing more mature cells.

Pathogen-specific host responses define distinct pneumonia endotypes in the human lung

This study identifies distinct pneumonia endotypes based on pathogen-specific host responses in the lung, linking them to clinical outcomes and suggesting personalized treatment strategies.

Deep immune profiling of the peripheral blood reveals disease- and sex-associated immune cell signatures in patients with systemic sclerosis

The paper identifies sex-associated immune cell signatures in systemic sclerosis patients using high-dimensional immune profiling of peripheral blood mononuclear cells.

Dysregulation of anti-Ro60 B cell autoreactivity in systemic lupus erythematosus

This study tracks Ro60-specific B cells in SLE patients and healthy donors, revealing defects in distal tolerance and heterogeneity in tolerance enforcement.

Low-self-reactive, circulation-biased blood regulatory T cells sense danger-associated nucleotides to restrain atherosclerosis

The paper identifies circulation-biased blood regulatory T cells that respond to danger-associated nucleotides to suppress atherosclerosis.

Mechanistic learning to predict and understand minimal residual disease

This paper develops a mechanistic learning framework to predict BCR::ABL1 fusion gene status and minimal residual disease using clinical data, finding that stem-like cell state is a strong predictor.

HLA-DR MODULATION AND PD-1/PD-L2 CHECKPOINT SIGNALLING DEFINE A MECHANISTIC POTENCY AXIS FOR MESENCHYMAL STROMAL CELL IMMUNOSUPPRESSION

This study establishes a standardized potency assay for mesenchymal stromal cells by linking HLA-DR modulation and PD-1/PD-L2 signaling to their immunosuppressive activity.

Lymphatic egress recycles tumor-experienced effector CD8 T cells to sustain immune surveillance

The paper identifies a lymphatic circuit that sustains stem-like CD8+ T cells by recycling tumor-experienced effector T cells, enhancing immune surveillance and ICB response.

Heat-Triggered Dormancy Release in Low-ROS Pollen Grains Reveals a Conserved Reproductive Reserve

The paper shows heat stress activates dormant low-ROS pollen, revealing a conserved reproductive reserve mechanism in plants.

Coincident epithelial signals restrain commensal-specific CD8αβ+ T cells in the intestine

The paper identifies a commensal-specific CD8αβ+ T cell population in the intestine driven by a single commensal species and shows how epithelial signals regulate their differentiation and restraint.

Circulating inflammatory ILC2s as a biomarker of gastric cancer progression

This study identifies circulating inflammatory ILC2s as a biomarker for gastric cancer progression, showing their association with disease severity and potential for early detection.

Cell-resolved high-dimensional imaging analysis, therapeutic modeling, and a Phase Ib clinical study validate BCL-2 as a target across heterogeneous CRPC subtypes

This study validates BCL-2 as a therapeutic target in CRPC by showing its upregulation under AR inhibition and demonstrating efficacy of BCL-2 inhibitors in preclinical and clinical models.

What Can We Count On? Performance of Microplate Cell Counting Assays in 2D Monolayer and 3D ECM-based In Vitro Tumour Models

This study evaluates three cell counting assays in 2D and 3D in vitro models, finding that 2D cultures yield more precise and reliable results than 3D ECM-based cultures.

Profiling co-occurrent morphological phenotypes and their degree of expression severity in vacuolated cells by holo-tomographic flow cytometry and fractal analysis

The paper introduces a pipeline to profile co-occurring morphological phenotypes in vacuolated cells using HTFC and fractal analysis, assigning severity levels to each phenotype.

CMV Replication Drives IFNγ-Mediated Sensitization of AML Cells to Cytotoxic Killing Through the NKG2C-HLA-E Axis

The paper shows CMV replication increases IFNγ, which makes AML cells more vulnerable to NK cell killing via the NKG2C-HLA-E pathway.

Evaluating the Utility of a Nanoscale Flow Cytometer for Detection of Surface Proteins on HIV and Extracellular Vesicles

This paper evaluates the CytoFLEX nano flow cytometer's performance for detecting surface proteins on HIV and extracellular vesicles, finding it has higher scatter sensitivity and detects smaller particles and EV populations compared to a conventional flow cytometer.

High-dimensional multiomics reveals perturbations to IL-6/IL-6R axis and RUNX3 in CD4+ T cells during third trimester pregnancy

This study identifies changes in CD4+ T cell surface proteins and transcriptomes during pregnancy, highlighting perturbations in the IL-6/IL-6R axis and RUNX3 expression.

Human lung γδ T cells maintain functionality during inflammatory lung disease

This study examines the functionality of human lung γδ T cells in inflammatory lung disease and finds they maintain polyfunctional cytokine expression despite environmental changes.

Maternal Inflammation in Late Gestation Alters Vaccine-Induced Immune Responses in Adult Murine Offspring

This study shows that maternal inflammation during late gestation in mice alters immune responses to vaccination in adult offspring.

Rapid and affordable generation of compensation beads for nanoscale flow cytometry

The paper describes a method to rapidly create affordable compensation beads for nanoscale flow cytometry compatible with various antibodies.

Monocyte Metabolic Plasticity and Cytokine Production Differentiate Latent TB Infection from Active Disease

This study compares monocyte phenotypes, function, and metabolism in latent TB infection, active TB disease, and healthy controls, finding distinct metabolic and cytokine profiles that differentiate the conditions.

Pre-Human Immunodeficiency Virus (HIV) infection Th17 CD4+ T cells as predictors of early HIV disease progression

The paper examines pre-HIV Th17 cell levels as predictors of early HIV disease progression, finding higher pre-infection Th17 frequencies correlate with faster CD4 decline.

FLASH Radiotherapy Mitigates Radiation-Induced Lymphopenia and Prevents Immunosuppression via Chk1-STAT3 Axis Modulation in a Preclinical Thoracic Irradiation Model

This study shows FLASH radiotherapy reduces lymphocyte apoptosis and prevents immunosuppression by modulating the Chk1-STAT3 pathway in a preclinical model.

Translational toolkit for reproducible, cross-study profiling of human ageing hallmarks in human blood and tissue

The paper develops a toolkit to measure multiple ageing hallmarks in human blood and tissue samples, identifying age-related changes in immune cells, senescence, and mitochondrial function.

Optimizing High Parameter T Cell Immunophenotyping Through Direct Comparison of Conventional and Spectral Flow Cytometry

This study compares conventional and spectral flow cytometry for high-parameter T-cell phenotyping, finding that spectral flow cytometry provides better resolution and sensitivity, especially with optimized panels.