multiome

Accelerated discovery of thermostable vaccines using data-efficient AI

This study uses AI to rapidly develop thermostable mRNA vaccines that maintain bioactivity at high temperatures and can be delivered via microneedle patches.

Engineered mRNA nanostructures expand the design space of mRNA therapeutics through programmable protein expression and immune stimulation

This paper introduces mRNA-OG, a programmable mRNA nanostructure that affects protein expression, delivery, and immune response through its architecture.

Beyond codon optimality: codon pairs regulate mRNA stability dependent on translation

The paper identifies codon pairs and amino acid pairs as regulators of mRNA stability in zebrafish, showing context-dependent decay mechanisms involving deadenylation and decapping.

The Virtual Biotech: A Multi-Agent AI Framework for Therapeutic Discovery and Development

The Virtual Biotech uses AI agents to integrate multi-omic and clinical data for drug discovery, identifying genomic features linked to trial success and proposing therapeutic strategies.

ERBB2 establishes the timing of progesterone priming required for uterine receptivity

This study shows ERBB2 controls the timing of progesterone responsiveness in the uterus, ensuring proper receptivity for implantation.

N6-methyladenosine promotes temozolomide resistance through non-canonical regulation of mRNA stability in glioblastoma cells

This study shows that m6A methylation stabilizes genes involved in temozolomide resistance in glioblastoma, and inhibiting METTL3 can restore drug sensitivity.

An intersectional expression platform for gene complementation using RNA-fragment end joining (REJ)

This paper introduces an RNA-fragment end joining (REJ) platform for gene complementation that enables precise and efficient splicing of RNA units into functional mRNAs.

Quantifying Uncertainty in Brain Age Predictions via Conformal Prediction

This paper introduces brain age intervals (BAIs) as a method to quantify uncertainty in brain age predictions using conformal prediction, showing stable coverage and highlighting the need for larger samples.

Mammalian spermatogenesis at single-cell resolution downstream of genetic perturbations in meiotic double-strand break repair

Single-nucleus RNA+ATAC profiling of 32 mice shows asymmetric PRDM9 binding drives asynapsis, pachytene cell loss, aneuploidy and multi-megabase crossover redistribution, tuned by a chromosome 15 locus.

scPyviewer: a Python-native interactive viewer from AnnData single-cell data

scPyviewer provides a Python-native interactive viewer for AnnData single-cell data, matching R Shiny tools' features and outperforming them in speed and memory efficiency.

A novel RRM activity orchestrates ribosome homeostasis

40S subunit binding to a previously unrecognised RRM in LARP1 activates its TOP-binding HEAT repeats, licensing repression of ribosomal protein mRNA translation in human cells.

ALS-associated exitron splicing produces UBQLN2 isoforms with distinct properties

The paper identifies an exitron in UBQLN2 that generates an alternatively spliced isoform with distinct properties linked to ALS.

Cyclic-thiourea ionizable lipids program multilamellar mRNA-LNPs for sustained expression, frozen-storage stability and potent vaccine immunotherapy

This paper introduces a cyclic-thiourea ionizable lipid (HI-62) that forms multilamellar mRNA-LNPs with sustained expression, stability, and potent vaccine immunotherapy effects.

Transition Intermediates Encode Ligand Efficacy at the μ-Opioid Receptor-Gi Protein Complex

The paper uses computational and experimental methods to identify transition intermediates in the μ-opioid receptor-Gi protein complex that encode ligand efficacy.

Associations between striatal neurochemical changes and resting-state functional connectivity following transcranial temporal interference stimulation

This study investigates whether 20-Hz tTIS modulates striatal neurochemical markers and functional connectivity, finding group-dependent associations between Glx changes and network alterations.

A root pathogen drives rhizosphere enrichment of antagonistic Pseudomonas and induces production of an antimicrobial metabolite

This study shows that a root pathogen activates antimicrobial production in beneficial bacteria, enhancing plant defense against itself.

RsmW is an iron-responsive 3 UTR derived small regulatory RNA that contributes to biofilm formation and virulence of Pseudomonas aeruginosa

This study identifies RsmW as an iron-responsive sRNA that enhances biofilm formation and virulence in Pseudomonas aeruginosa under iron limitation.

Immune Aging is an Independent Risk Factor for Cardiovascular Disease

The paper introduces IMM-AGE, a metric of immune-aging, and shows it independently predicts cardiovascular disease risk across multiple cohorts.

SCALE: Scalable Conditional Atlas-Level Endpoint transport for virtual cell perturbation prediction

A set-aware latent transport model predicts treated cell populations from unpaired controls, keeps perturbation-specific signal in CRISPR, drug and cytokine data, and guided a cytokine choice tested in three donors.

MIND the gap: methodological considerations and guidance for structural MRI similarity network analysis with MIND

The paper provides methodological guidance for using MIND, a structural MRI similarity metric, and demonstrates how input properties affect its accuracy using simulated and observational data.

Observations of anisotropic paramagnetic and diamagnetic susceptibility in the primate brain

This study uses MRI and computational methods to show that paramagnetic substances contribute to susceptibility anisotropy in primate brain tissue, alongside diamagnetic components.

A geometric anthropomorphic phantom for quantitative susceptibility mapping: accuracy and repeatability

This paper evaluates the accuracy and repeatability of quantitative susceptibility mapping using an anthropomorphic phantom, finding better performance at 3T than 7T.

immgenT: A Comprehensive Reference of Convergent T-cell States in the Mouse

immgenT creates a comprehensive T-cell molecular atlas across mouse organs and disease states, identifying 8 lineages and 107 clusters using deep generative models and integrating external datasets.

5-azacytosine induces cytotoxicity via 5-methylcytosine depletion on chromatin-associated RNA in leukemia

This paper shows that 5-azaC induces leukemia cell death by depleting m5C in chromatin-associated RNA, leading to transcriptional repression and disrupting key regulatory pathways.

Glioblastoma Tumors with Decelerated Epigenetic Aging Are Characterized by Glutamatergic Neuronal Activity and Stemness

This study identifies decelerated epigenetic aging in glioblastoma tumors linked to glutamatergic neuronal activity and stemness, showing that anti-glutamatergic drugs enhance temozolomide's effectiveness.

Melodic Modulation of Pain and Cognition: Neurobehavioral Effects of Indian Instrumental Music in Mice

This study investigates the effects of Indian instrumental music on pain and cognition in mice, finding significant reductions in pain and anxiety along with neurochemical changes.

Spatio-temporally regulated maternal RNA decay factor essential for zebrafish development

The paper identifies Cth1 as a spatio-temporally regulated maternal RNA decay factor essential for zebrafish development, showing its role in promoting deadenylation and decay of maternal mRNAs.

In vivo multimodal lineage tracing of mammalian development by DeepTrack barcoding

The paper introduces DeepTrack, a lineage tracing model that combines cellular barcoding with single-cell multi-omics to study cell fate transitions and molecular changes during mammalian development.

Longitudinal Subject Pairing in Cross-Sectional Neonatal Data Reveals Asynchronous Structural and Functional Brain Maturation

The paper uses a longitudinal subject-pairing framework on cross-sectional neonatal data to reveal asynchronous structural and functional brain maturation, finding a functional maturation lag of about five days relative to structural development.

A multi-b-value test-retest diffusion MRI brain dataset for model validation and reproducibility assessment

The paper introduces a densely sampled longitudinal diffusion MRI dataset for validating dMRI techniques and assessing reproducibility.