DNA Repair Mechanisms

This study investigates how DNA polymerase interacts with the replication fork under stress and finds that prolonged stress inactivates replication and locks the fork, which is reversible only by helicase.

The paper identifies the tetrameric INTS6-SOSS1 complex as essential for DNA:RNA hybrid autoregulation at double-strand breaks, facilitating DNA repair through PP2A recruitment and DARTs prevention.

This paper revises the mechanism of hydroxyurea-induced cell cycle arrest in yeast, showing it is ROS-mediated rather than Mrc1-dependent, and introduces an improved alternative to HU called RNR-deg.

This study uses molecular dynamics simulations to investigate how PARP-1's Zn1 and Zn2 domains interact with DNA single-strand breaks, revealing their cooperative role in SSB recognition.

This study shows that 53BP1 deficiency increases BIR-mediated hyperrecombination, leading to genome instability and potential cancer treatment opportunities.

This study identifies JMJ27 as a histone demethylase that modulates H3K9me2 levels during UV-induced photodamage repair in plants.

The paper identifies that TMEJ is optimally licensed during mitotic commitment and is regulated by PLK1, with DNA2 deficiency impairing TMEJ by delaying S-phase progression.

The paper shows that inactive PARP2 causes Tp53-dependent lethal anemia by blocking replication-associated nick ligation in erythroblasts, explaining PARPi-induced hematological toxicities.

This study identifies the base excision repair pathway as a regulator of transcription-replication conflicts in pancreatic ductal adenocarcinoma, showing that its inhibition enhances DNA damage and reduces cell proliferation.

The paper identifies FIGL1 as a key factor limiting non crossover events in Arabidopsis thaliana meiosis through tetrad sequencing analysis.

This study reports the structural basis of how BRCA2's PhePP motifs stabilize RAD51 and DMC1 nucleoprotein filaments through a conserved interaction mode.

This study presents six high-resolution structures of the E. coli clamp loader complex (CLC) during the clamp loading process, revealing the molecular mechanism of clamp opening and DNA binding.

This study identifies ELOF1 as a critical factor for somatic hypermutation and class switch recombination by facilitating RNAPII pausing and AID targeting.

The paper reports cryo-EM structures of Spo11 core complexes bound to DNA, revealing DNA-binding specificity and structural variation in homologs.

This study identifies a mechanism where HTT recruits BRG1 and NHEJ components to repair transcription-associated DSBs in neurons, and shows that mutant HTT impairs this process, contributing to HD pathology.

The paper identifies Ubp10's role in facilitating Okazaki fragment maturation by promoting PCNA dissociation in budding yeast.

This study identifies USP50 as a ubiquitin-binding protein that regulates helicase and nuclease activities during DNA replication to prevent fork collapse and maintain genomic stability.

This study shows that NuRD chromatin remodeling is essential for repairing exogenous DNA damage in C. elegans germline, with defects leading to reduced fertility and aneuploidy.

This study identifies RAD54L's distinct roles in two RAD51-mediated replication fork reversal pathways, showing it is critical in the HLTF/SMARCAL1 pathway but not in the FBH1 pathway.

This study shows SMARCAD1 resolves R-loops at replication forks, preventing genome instability and cancer mutations.

This study shows NSD2 has dose-dependent effects on DSB repair pathways, repressing non-c-NHEJ and promoting c-NHEJ while not affecting HR or total NHEJ efficiency.

This study identifies how CSB enhances SNM1A nuclease activity during ICL repair by interacting with its nuclease core and altering substrate conformation.

This study identifies phosphorylation events on WRN that regulate end-resection and RAD51-mediated repair at replication forks.

This study investigates the role of shieldin and CST in DNA end-joining and HR suppression, showing their collaboration in DNA repair and the independence of 53BP1's effects.

The paper shows that RAD18 inactivation in HCT116 cells increases recombination and reduces TLS activity, linked to PCNA K164 ubiquitination, but not SUMOylation.

The paper investigates the role of yeast EndoG (Nuc1) in preventing genome instability by degrading cytoplasmic DNA and regulating its transfer to the nucleus.

The paper shows that RPB1 degradation by the ubiquitin-proteasome system occurs in trans and is controlled by NER activity, regardless of lesion recognition mechanism.

This study identifies STK19 as a critical factor in human transcription-coupled repair, linking CSA, UVSSA ubiquitination, and TFIIH loading.

This study shows PKA promotes NHEJ over resection in DSB repair by interacting with 53BP1, highlighting PKA's role in DNA damage response and genome stability.

The paper identifies ANLN's nuclear role in UV-induced translesion synthesis by interacting with PCNA to promote DNA repair and genomic stability.