Advanced biosensing and bioanalysis techniques

This study explores optimizing aptamer-drug conjugates for GBM treatment by focusing on internalization and tumor homing through in vivo SELEX, but faces challenges with tumor specificity.

This paper demonstrates the use of colloidal DNA/RNA nanoaggregates for data storage and obfuscation by encoding digital information into oligo libraries and manipulating ionic strength.

The paper introduces SPARXS-Hyb, a method for multiplexed screening of DNA hybridization kinetics, identifying sequences with improved performance in DNA-PAINT.

This paper develops a nuclease-stable L-RNA glycine sensor using cross-chiral ligation, enabling extracellular glycine detection in serum.

The paper introduces logicFRIES, a fluorogenic RNA platform enabling eight-plex live-cell imaging through logic-gated activation and sequential fluorescence imaging.

This paper introduces a cell-based sensor called LUNAR that detects extracellular double-stranded DNA with nanomolar sensitivity in mammalian cells.

This paper introduces a method to detect multiple molecular classes using nanopore sequencing by encoding targets into periodic DNA barcodes, achieving high specificity and error tolerance.

This paper introduces a DNA origami nanomechanical amplifier that translates molecular conformational changes into measurable mechanical displacements, enabling single-molecule analysis of binding events without labeling or fluorescence.

This paper develops a high-throughput platform to rapidly adapt DNA aptamers against evolving SARS-CoV-2 variants, identifying mutants with improved binding affinity and specificity.

This paper introduces dcORBIT, a method that extends DNA rotation tracking during transcription to over 10 minutes at 20 Hz with single base-pair precision.

This paper develops a CRISPR/Cas12a-based system for sensitive and multiplexed detection of EV-derived microRNAs using DNAzyme and machine learning for cancer classification.

This study investigates gene-length DNA monolayers on gold electrodes for cell-free protein expression, showing tunable surface density and partial functional retention under certain conditions.

OrthoSeq designs DNA sequence-pair libraries with thermodynamic orthogonality, outperforming sequential methods in library size under constraints.

This paper introduces an automated design method for stiffness-tunable DNA origami nanostructures that can be manufactured into functional metamaterials.

This paper introduces molecular pendulum electrochemical sensors with active-reset mechanisms for real-time, ultrasensitive protein detection in biofluids.

This paper introduces an enzyme-free DNA-based templated dimerization reaction controlled by a fuel strand that displaces product to overcome inhibition, enabling information propagation from templates.

This paper identifies high-performance split sites in DNA aptamers through large-scale mutational analysis, improving signal-to-background ratio for RNA detection.

This study develops and tests DNA aptamer-drug conjugates (ApDCs) for glioblastoma treatment using an in vivo SELEX approach in a patient-derived xenograft model.

The paper identifies DNA-DNA kissing complexes and develops an adenosine-triggered aptaswitch using them for controlled nanostructure assembly.

This paper demonstrates the co-assembly of DNA nanotubes and condensates in synthetic compartments, enabling programmable structures that mimic cellular organization.

This paper develops a computational framework to design crisscross DNA megastructures, optimizing handle assignment and expanding handle libraries to enable large-scale fabrication.

This paper presents a method to achieve programmable and dynamic DNA localisation at synthetic cell membranes using RNA linkers and enzymatic degradation.

This paper develops an optical nanosensor array to detect anthracycline chemotherapeutics in synthetic biofluids using machine learning for classification and concentration analysis.

AptBacterialDB is a manually curated database of antibacterial aptamers with detailed annotations and integration tools to support research and development.

AptCancerDB curates experimentally validated anticancer aptamers, providing a resource for discovery and development of diagnostics and therapeutics.

This paper demonstrates that transcription-induced coacervation accelerates and enhances cell-free biosensing by mimicking intracellular phase separation, improving sensitivity and reducing detection time.

This paper develops an impedimetric aptasensor using DNA tetrahedral nanostructures for ultrasensitive IL-6 detection with a limit of detection of 55 ag/mL.

This paper introduces a computational pipeline using DNA k-mer imprints to agnostically classify and distinguish materials based on their sequence patterns.

This paper introduces an enzyme-free DNA strand displacement network that uses DNA templates to assemble non-covalent DNA multimers under isothermal conditions, achieving far-from-equilibrium product formation.

This paper develops DNA-RNA artificial cells with RNA organelles that enable spatially encoded functions and signal processing through aptamer-based protein recruitment and environmental responsiveness.