William M. Shih

This paper develops a computational framework to design crisscross DNA megastructures, optimizing handle assignment and expanding handle libraries to enable large-scale fabrication.

This study uses single-molecule imaging to show that Parkinson's disease-associated alpha-synuclein aggregates are more stable than physiological multimers under dilution.

This study shows that lung-targeted delivery of immunostimulatory dsRNA via LNPs enhances systemic cytokine responses and antitumor activity in melanoma models.

This study validates DoriVac, a DNA origami vaccine, as an effective treatment for metastatic melanoma in mouse models, showing reduced tumor nodules and enhanced immune activation when combined with PD-L1 blockade.

This study demonstrates that DNA origami vaccine nanoparticles enhance both humoral and cellular immune responses against infectious diseases like SARS-CoV-2, HIV, and Ebola.

The paper introduces a DNA nanoswitch calipers platform to simultaneously measure structural and mechanical properties of biomolecules, demonstrating its application on telomeric DNA G-quadruplexes.

This paper demonstrates that reusing excess staple oligonucleotides with replenishment can reduce DNA origami production costs by up to 80%.

The paper quantifies cargo on DNA origami nanoparticles using gel-based and fluorescence-based methods for therapeutic applications.

This study developed a DNA origami-based cancer vaccine that co-delivers antigen and CpG immune adjuvant with an optimal nanospacing for Th1 immune polarization.

Crisscross polymerization of DNA-origami slats enables the creation of micron-scale structures with uniquely addressable nanoscale features.

This paper introduces a method for robust nucleation control in DNA-based self-assembly using crisscross polymerization, achieving strictly seed-initiated growth without spontaneous nucleation.

This paper introduces a methanol-responsive polymer (MeRPy) that selectively binds and releases DNA targets, improving NGS library quality by depleting unwanted transcripts.

The paper demonstrates the use of nucleic-acid-based transcription factors to implement complex transcriptional logic in cell-free systems.

This paper describes a method for producing high-purity single-stranded DNA for various applications.