Nuclear Structure and Function

Acetylation facilitates NPM1 anchoring in the nucleolus by boosting liquid-liquid phase separation

This study shows that acetylation of NPM1 at Lys27 and Lys54 promotes its anchoring in the nucleolus by enhancing liquid-liquid phase separation.

DNA damage at Lamina-Associated Domains triggers nuclear envelope reorganization and chromatin detachment to prevent nuclear envelope blebbing and genome instability.

The physical state of the nucleus tracks its function across eukaryotes

The paper investigates the relationship between nuclear density and function across eukaryotes, finding that nuclear import, not transcription, determines density in some organisms.

Abnormal nuclear organization impairs adipogenic lineage commitment and terminal maturation in Hutchinson-Gilford progeria syndrome (HGPS)

This study shows that HGPS disrupts adipogenic lineage maturation through chromatin reorganization defects, despite normal lineage commitment rates.

Progerin disrupts intestinal epithelial homeostasis by impairing mitochondrial metabolism and absorptive enterocyte maturation

Differential mRNA localization of karyopherin-β2 homologs in C. elegans and humans

The paper compares mRNA localization of karyopherin-β2 homologs in C. elegans and humans, finding that human orthologs do not localize to the nuclear envelope like their C. elegans counterpart.

Reduced emerin expression marks gastric cancer progression and invasive nuclear remodeling

This study shows reduced emerin expression correlates with gastric cancer progression and nuclear remodeling, similar to findings in breast cancer.

Hierarchical TPR:ZC3HC1 interactions drive stepwise assembly of serially arrayed nuclear basket appendages extending into vertebrate cell nuclei

Nuclear wrinkles result from geometric adaptations to cellular and nuclear morphological changes in epithelial cells

The paper investigates nuclear wrinkles in epithelial cells, finding they form due to geometric adaptations to mechanical stress and cell shape changes, without causing damage.

Senescence-induced Spatio-temporal Re-organization of the Nuclear Lamina Dynamic Landscape at Single-molecule Level in Live Human RPE Cells

This study investigates how senescence alters the spatio-temporal dynamics of the nuclear lamina at the single-molecule level in human RPE cells.

Molecular Force Gates Regulate Mechanical Transmission Across the Nuclear Envelope

The paper identifies force gates in the LINC complex using molecular dynamics simulations, showing how specific molecular contacts regulate mechanical force transmission across the nuclear envelope.

The scaffold nucleoporin Nup188 undergoes liquid-liquid phase separation

This study shows that the scaffold nucleoporin Nup188 can form liquid-liquid phase separation condensates, and Nup93 binding suppresses this process.

TMEM209/NPP-28 partitions PLK-1 between the nuclear envelope and nuclear interior during mitosis

This study identifies NPP-28 as a regulator of PLK-1 localization during mitosis, showing it partitions PLK-1 between the nuclear envelope and interior to control NEBD.

Nucleolar buffering of lamina-associated heterochromatin restrains Progerin-induced ageing programs

This study shows that the nucleolus buffers Progerin-induced ageing by sequestering lamina-associated domains, preserving heterochromatin integrity and preventing transcriptional ageing.

How the TREX-2 complex associates with the nuclear pore

The paper reports an extended structure of the human nuclear pore complex, revealing TREX-2 components as integral parts of the nuclear ring.

A novel FRET-force biosensor for nucleoporin gp210 reveals that the nuclear pore complex experiences mechanical tension

The paper develops a FRET-force biosensor to measure mechanical tension on the nuclear pore complex and finds that nuclear strain, not cytoskeletal forces, primarily drives these forces.

Emerin modulation impacts viability, proliferation, migration, and DNA repair signaling in cisplatin-treated glioblastoma cells

This study shows that modulating EMD levels in wild-type p53 GBM cells affects cisplatin response, viability, proliferation, and DNA repair signaling.

Cytokine-induced nuclear translocation of STAT1 via a non-transferable NLS

This study identifies a non-transferable NLS in pSTAT1 that requires importin 5, Ran-GTP, and CAS for nuclear translocation and release, revealing a novel mechanism for cytokine-induced STAT1 signaling.

Remodeling of cholesterol metabolism in melanoma progression regulates nuclear membrane mechanics.

This study shows that cholesterol metabolism remodeling in melanoma progression alters nuclear membrane mechanics, increasing tension and vulnerability to rupture during cellular confinement.

Spatial control of karyopherin binding avidity within NPC mimics revealed by designer FG-Nucleoporins

This study designs and tests artificial FG-Nups to show that spacing and charge-to-hydrophobicity ratio affect Kap95 binding and transport selectivity through NPCs.

Expandable DNA-origami rings as a nanomechanical platform for studying disordered nucleoporins

This study uses DNA-origami rings to investigate FG-Nup interactions and their impact on NPC function, revealing how sequence and post-translational modifications affect protein behavior.

In-cell structures visualize human pre-ribosome assembly in the nucleolus

This study visualizes human pre-ribosome assembly in nucleoli using cryogenic correlative light and electron tomography, revealing structural states and interactions.

Cancer-associated nucleolar morphological changes coincide with altered subcompartment organization and accelerated nascent rRNA trafficking in breast cancer cells

This study compares nucleolar morphology, subcompartment organization, and rRNA dynamics between normal and breast cancer cells, showing that cancer-associated changes are reversible and involve multiple dimensions.

NEMP1 organizes the meiotic telomere LINC interface to control chromosome movement and telomere integrity

This study identifies NEMP1 as a critical organizer of meiotic telomere-nuclear envelope coupling and chromosome dynamics in mouse oocytes, showing its role in telomere integrity and genome stability.

Cardiomyocyte vulnerability to lamin polymer disruption revealed by saturation mutagenesis

The paper identifies cardiomyocyte-specific vulnerabilities to LMNA mutations using saturation mutagenesis in hiPSCs and cardiomyocytes, revealing how lamin polymer disruption leads to disease.

Multivalent lamin binding controls the meshwork structure in a self-assembled model of the nuclear lamina

This study uses a polymer-physics model to show how lamin binding parameters control nuclear lamina structure and mechanopathology.

Genomic splice-donor disruption rescues systemic disease in progeria

This paper shows that disrupting a faulty splice donor site in progeria reduces toxic protein levels and extends lifespan in mice.

Endogenous Nuclear Desmin Associates with the Nuclear Envelope in Human Skeletal Muscle Cells

The paper identifies endogenous desmin's association with the nuclear envelope in human skeletal muscle cells and suggests its role in nucleo-cytoskeletal communication and nuclear architecture during myogenesis.

Evolutionary diversification of the nuclear pore complex in Entamoeba histolytica reveals conserved and lineage-specific nucleoporins

This study identifies and characterizes nucleoporins in Entamoeba histolytica, revealing both conserved and lineage-specific features of the nuclear pore complex.

Atypical RanGAP drives nucleocytoplasmic transport in a parasitic Alveolate

A RabGAP-fold protein, TBC9, supplies the missing RanGAP activity in Toxoplasma; its acidic C-terminal motif promotes Ran binding and marks TBC9 orthologs across non-ciliate alveolates.