Barrier Structure and Function Studies

The paper examines how endothelial cell structure influences capillary diameter heterogeneity in mouse brain microvasculature using electron microscopy data.

The paper uses molecular dynamics simulations to study how pathogenic and artificial mutations affect ion selectivity in Claudin-5 paracellular pores, identifying structural models that align with experimental observations.

This study uses 3D electron microscopy to examine the neurovascular unit, revealing new structural roles for precapillary sphincters, collagen, and astrocytic endfeet in neurovascular coupling.

This study investigates how alpha-synuclein aggregates affect blood-brain barrier integrity in vitro using co-cultures of brain endothelial cells with neurovascular unit components.

The paper identifies epigenetic and signaling factors that regulate BBB formation and maintenance in CNS endothelial cells.

This study identifies distinct endocytic pathways for Aβ40 and Aβ42 in BBB endothelial cells, showing clathrin-mediated uptake for Aβ40 and caveolae-mediated uptake for Aβ42, with differential intracellular trafficking outcomes.

The paper develops anti-mTfR VHHs for BBB transcytosis and evaluates their hypothermic effects and target engagement in a mouse model, finding no direct correlation between hypothermia and plaque labeling.

This study shows RFC1 is present in retinal microvessels and regulates inner blood-retinal barrier integrity by affecting tight junction proteins and collagen-4.

This study shows that mitochondrial complex I regulates blood brain barrier permeability by controlling energy production and metabolic flux in endothelial cells.

The paper identifies 17β-estradiol and CAT-1 as regulators of leptin transport across an iPSC-derived blood-brain barrier model.

The paper examines capillary density in the mouse brain and finds it is mostly homogeneous, with energy supply per neuron decreasing as neuronal density increases.

This study shows that disrupting glycogen utilization in mice causes microvascular constrictions and worsens ischemia outcomes.

This study compares two structural models of Claudin-5 complexes in BBB tight junctions using molecular dynamics and free energy calculations to assess their ionic transport properties.

The paper demonstrates that oligodendrocytes mediate axo-vascular coupling in the mouse optic nerve by regulating vascular dilation in response to axonal activity.

The paper identifies Wnt-β-catenin signaling repression as key to arachnoid barrier cell specification in mouse meninges and shows its role in preventing pathogen entry during development.

This study shows that p-cresol glucuronide, a host-gut microbial co-metabolite, reduces blood-brain barrier permeability and modulates brain transcriptome expression in mice.

This study shows that pericyte loss in aged brains leads to capillary dilation, flow heterogeneity, and structural disruption, highlighting pericyte remodeling as a therapeutic target.

This study uses a SARS-CoV-2 biomimetic to investigate how BBB dysregulation may lead to neuroinflammation without direct viral infection.

The paper uses two-photon microscopy to show that S1P signaling impairment causes glycocalyx loss in arterioles but not other vessel types in Apom-/- mice.

The paper presents a meta-analysis of electron microscopy datasets to explore cerebrovascular structures and identifies features important for vascular function and segmentation improvements.

The paper demonstrates that brain capillary endothelial cells and pericytes express functional KATP channels that regulate membrane potential and cerebral blood flow through adenosine signaling.

This study shows that isoflurane induces initial calcium increases then suppression in cerebrovascular endothelial cells, disrupting vascular tone during anesthesia.

This study identifies capillary pericytes as metabolic sentinels that increase blood flow in response to glucose depletion through KATP channel activation.

This study shows that tunneling nanotubes (TNTs) enable mitochondrial transfer between BBB cells, rescuing astrocytes from ischemia/reperfusion-induced apoptosis and highlighting pericytes' role in BBB preservation.

This study shows that Norrin/β-catenin signaling in mural cells increases Lama2 expression, which promotes neurovascular unit assembly and barrier maturation.

The paper identifies cell-type-specific molecular changes in cerebrovascular cells across brain regions in Alzheimer's Disease, linking them to genetic variants and potential therapeutic targets.

The paper maps the spatial relationship between cerebrovasculature, pericytes, and neuronal cell types in mice to reveal how they support regional brain energy homeostasis.