Transgenic Plants and Applications

This paper demonstrates the use of bacteriophage P22 virus-like particles as nanoscale protein scaffolds for plant synthetic biology, showing their ability to assemble, encapsulate, and display proteins in Nicotiana benthamiana.

This study shows that eliminating polyphenol oxidase in Nicotiana benthamiana improves recombinant protein yield, purity, and native-state integrity during extraction.

This study identifies a 79-nucleotide sequence from the Arabidopsis CYSTM1 5'UTR that significantly enhances transgene expression in plants and bacteria.

The paper models variability in Nicotiana benthamiana transient expression and provides guidelines for minimizing it through experimental design and power analysis.

This study shows that knocking out two polyphenol oxidase genes in Nicotiana benthamiana improves recombinant protein purification by reducing aggregation and increasing yields.

This study combines plant and in vitro glycoengineering to produce homogeneous glycoforms of pharmaceutical proteins efficiently.

This paper produces prolyl-hydroxylated human type III collagen in moss bioreactors, achieving 1 mg/L in 11 days with no need for heterologous P4H expression.

This study develops a GMP-compliant manufacturing process for TBSV-based nanoparticles designed to induce immune tolerance in autoimmune diseases and evaluates its scalability and environmental impact.

This paper develops a high-throughput platform for transient and stable multigene expression in Arabidopsis suspension cells, enabling efficient protein production for synthetic biology applications.

This study evaluates three transgene expression constructs in lettuce using the RUBY reporter, finding that the LsUBI promoter/terminator provides stable, uniform expression while the AtUBI promoter/tRBCS offers an intermediate option.

The paper introduces a cost-effective, one-step protein purification method for plants using a DIY GFP-Trap-protease system, enabling rapid isolation of soluble and membrane-bound proteins.

The paper develops a modular geminivirus-based vector system for improved protein expression and gene targeting in plants, showing that native promoters and removal of virion-sense genes enhance reporter expression without affecting replicon accumulation.

This paper produces and purifies human papillomavirus type 16 virus-like particles in Physcomitrella photobioreactors, achieving high yields and successful assembly.

This paper presents a protocol for purifying recombinant proteins from Nicotiana benthamiana for structural studies, demonstrating its effectiveness for complex purification and cryo-EM analysis.

This study shows that expressing a constitutively active nitrate reductase in Nicotiana benthamiana increases SARS-CoV-2 Spike protein production by reducing leaf senescence and improving nitrogen metabolism.

This study successfully produces recombinant spider silk protein containing both N- and C-terminal domains in moss photobioreactors.

This paper evaluates Chlamydomonas incerta as a platform for recombinant protein production, showing higher expression levels for mCherry and comparable performance for xylanase and PHL7 compared to C. reinhardtii.

This paper shows that Rhizobium rhizogenes A4-derived strains provide more efficient transient gene expression in Nicotiana benthamiana and other solanaceous plants compared to common agrobacterial strains.

The paper shows that localizing enzymes to biomolecular condensates in Nicotiana benthamiana increases enzyme stability and improves metabolic pathway yields.

The paper compares immune responses in Nicotiana benthamiana leaves expressing coronavirus-like particles (CoVLPs) or influenza VLPs, showing overlapping defense responses and demonstrating that Kunitz trypsin inhibitors reduce CoVLP-induced symptoms.

The paper identifies immunity genes that suppress transient protein expression in Nicotiana benthamiana by silencing them and observing increased GFP expression.

This study demonstrates that viral vectors can deliver and stably express recombinases in plants, enabling heritable genetic modifications and lineage tracing.

This study develops a method to deliver proteins into plant nuclei by partially digesting the cell wall, demonstrating efficient GFP delivery in Arabidopsis root cells.

The paper introduces CuBe, a copper-regulated plant-based expression system for scalable recombinant protein production, demonstrating high eGFP and anti-SARS-CoV-2 antibody expression in Nicotiana benthamiana.

This paper develops a copper-inducible gene expression system in Nicotiana benthamiana to tightly regulate transgene expression and cell death assays.

The paper develops a chloroplast-based cell-free gene expression platform across multiple plant species to accelerate synthetic biology research and gene expression studies.

This study produces biologically active human basic Fibroblast Growth Factor (hFGFb) in transplastomic tobacco plants and demonstrates its biological activity.

The paper shows that SBT5.2 subtilases degrade the IgG antibody 2F5 in Nicotiana benthamiana apoplast, and their knockout increases 2F5 accumulation without affecting plant growth.

This study shows that Nicotiana benthamiana can effectively clone plant gene coding sequences by transcribing and splicing gDNA from certain species.

This study expresses glycoengineered pentameric SARS-CoV-2 IgMs in plants, showing 390-fold increased neutralization potency compared to IgG1 orthologues.