This study shows that Tpm isoforms in yeast bind actin filaments independently of formin isoforms and exhibit functional redundancy in actin cable maintenance.

bioRxiv · Apr 4, 2024 · PreprintCell Biology

Injectable butyrate-prodrug micelles induce long-acting immune modulation and suppress autoimmune arthritis in mice

S. Cao, E. Budina, R. Wang, M. Sabados, A. Solanki, M. Nguyen, K. Hultgren, A. Dhar, J. Hubbell

This study develops butyrate-prodrug micelles that target lymphatics to induce long-term immune modulation and reduce arthritis in mice.

bioRxiv · Aug 20, 2023 · PreprintBioengineering

Suppression of autoimmune arthritis and neuroinflammation via an amino acid-conjugatedbutyrate prodrug with enhanced oral bioavailability

S. Cao, E. Budina, M. M. Raczy, A. Solanki, M. Nguyen, T. N. Beckman, J. W. Reda, K. Hultgren, P. Ang, A. J. Slezak, L. A. Hesser, A. T. Alpar, K. C. Refvik, L. S. Shores, I. Pillai, , J. A. Hubbell

This study develops a butyrate prodrug, SerBut, which improves oral bioavailability and reduces autoimmune arthritis and neuroinflammation in mouse models.

bioRxiv · Apr 28, 2023 · PreprintBioengineering

ALIBY: ALFA Nanobody-Based Toolkit for Imaging and Biochemistry in Yeast

D. Akhuli, A. Dhar, A. S. Viji, B. Bhojappa, S. Palani

ALIBY is a yeast-specific toolkit that enables live-cell imaging and biochemical applications using the ALFA tag/NbALFA system, offering improved signal-to-noise ratio and versatility.

bioRxiv · Jul 18, 2022Cell Biology

mNeonGreen-tagged fusion proteins and nanobodies reveal localization of tropomyosin to patches, cables, and contractile actomyosin rings in live yeast cells

T. Hatano, T. C. Lim, I. Billault-Chaumartin, A. Dhar, Y. Gu, T. Massam-Wu, S. Adhishesha, L. Springall, W. Scott, L. Sivashanmugam, M. Mishima, S. G. Martin, S. Oliferenko, S. Palani, M. K. BALASUBRAMANIAN

The paper develops mNeonGreen-tagged tropomyosin and nanobodies to visualize tropomyosin localization in yeast, revealing new structures and dynamics in actin cytoskeleton.

bioRxiv · May 19, 2022Cell Biology

IntAct: a non-disruptive internal tagging strategy to study actin isoform organization and function

M. C. van Zwam, A. Dhar, W. Bosman, W. van Straaten, S. Weijers, E. Seta, B. Joosten, S. Palani, K. van den Dries

The paper introduces IntAct, a non-disruptive internal tagging strategy for studying actin isoform organization and function in mammalian and yeast cells.

bioRxiv · Oct 25, 2021 · PreprintCell Biology

Functional development of a V3-specific broadly neutralizing antibody isolated from a case of HIV superinfection

M. M. Shipley, V. Mangala Prasad, L. E. Doepker, A. S. Dingens, D. K. Ralph, E. Harkins, A. Dhar, D. Arenz, V. Chohan, H. Weight, K. Mandaliya, J. D. Bloom, F. Matsen, K. K. Lee, J. M. Overbaugh

This study characterizes the development of a V3-specific broadly neutralizing antibody from an HIV superinfection case, revealing unique epitope recognition and escape mechanisms.

bioRxiv · Mar 15, 2021Microbiology

Development of antibody-dependent cell cytotoxicity function in HIV-1 antibodies

L. E. Doepker, S. Danon, E. Harkins, D. Ralph, Z. Yaffe, A. Dhar, C. Wagner, M. Stumpf, D. Arenz, J. A. Williams, W. Jaoko, K. Mandaliya, K. K. Lee, F. A. Matsen, J. Overbaugh

This study reconstructs the developmental pathways of HIV-specific ADCC antibodies and identifies mutations required for antigen binding and ADCC function.

bioRxiv · Sep 24, 2020 · PreprintImmunology

Rapid development of an infant-derived HIV-1 broadly neutralizing antibody lineage

C. A. Simonich, L. Doepker, D. Ralph, J. A. Williams, A. Dhar, Z. Yaffe, L. Gentles, C. T. Small, B. Oliver, V. Vigdorovich, V. Mangala Prasad, R. Nduati, D. N. Sather, K. K. Lee, F. A. Matsen, J. Overbaugh

This study reconstructs the developmental lineage of an infant-derived HIV-1 broadly neutralizing antibody, showing a rapid evolution with fewer mutations compared to adult antibodies.

bioRxiv · Sep 13, 2018Immunology