Author

D. C. Stephens

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Cell Biology· 3 Biophysics· 2 Physiology· 1

Signature Assays, Organisms & Methods

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Research Focus:cell type annotation· 6 Approach:experimental assay· 6 Organism:mouse· 6 Assay:electronic health records· 2 Organism:human· 2 Assay:imaging· 2 Research Focus:protein folding & dynamics· 2 Assay:proteomics· 2

Frequent Co-Authors

A. Crabtree· 6 shared A. G. Marshall· 6 shared E. Garza-Lopez· 6 shared H. K. Beasley· 6 shared J. A. Gaddy· 6 shared J.-q. Shao· 6 shared K. Neikirk· 6 shared L. Vang· 6 shared

Activity by Year

2022-2023 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 45 mutual co-author links

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6 shared papersCell Biology

A. Crabtree

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Author profile →

6 papers

Recent·Most cited

Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation

D. C. Stephens, M. Mungai, A. Crabtree, H. K. Beasley, E. Garza-Lopez, L. Vang, K. Neikirk, Z. Vue, N. Vue, A. G. Marshall, K. Turner, J.-q. Shao, B. Sarker, S. A. Murray, J. A. Gaddy, , A. Hinton

The paper describes a method to isolate skeletal muscle from mice, generate satellite cells for culture, and validate cell populations using immunofluorescence.

bioRxiv · May 20, 2023Cell Biology

Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells

D. C. Stephens, A. Crabtree, H. K. Beasley, E. Garza-Lopez, K. Neikirk, M. Mungai, L. Vang, Z. Vue, N. Vue, A. G. Marshall, K. Turner, J.-q. Shao, S. A. Murray, J. A. Gaddy, C. Wanjalla, , A. Hinton

This paper describes a protocol for using proximity ligation assays (PLA) to study protein-protein interactions in mitochondria-endoplasmic reticulum contact sites (MERCs) in mouse skeletal muscle tissue and cells.

bioRxiv · May 20, 2023Cell Biology

Creating Optimal Conditions for OPA1 isoforms by Western Blot in Muscle Tissue

D. C. Stephens, A. Crabtree, H. K. Beasley, E. Garza-Lopez, M. Mungai, K. Neikirk, L. Vang, Z. Vue, N. Vue, A. G. Marshall, K. Turner, J.-q. Shao, S. A. Murray, J. A. Gaddy, C. Wanjalla, , A. Hinton

This paper presents an optimized western blot protocol to isolate and detect five OPA1 isoforms in muscle tissue.

bioRxiv · May 20, 2023Cell Biology

Ablation of Sam50 is associated with fragmentation and alterations in metabolism in murine and human myotubes

B. Shao, M. Killion, A. Oliver, C. Vang, F. Zeleke, K. Neikirk, Z. Vue, E. Garza-Lopez, J.-q. Shao, M. Mungai, J. Lam, Q. Williams, C. Altamura, A. Whiteside, K. Kabugi, , A. Hinton

This study shows that Sam50 deficiency in myotubes leads to mitochondrial fragmentation, altered metabolism, and impaired oxidative capacity in both mice and humans.

bioRxiv · May 20, 2023Physiology

Three-Dimensional Mitochondria Reconstructions of Murine Cardiac Muscle Changes in Size Across Aging

Z. Vue, K. Neikirk, L. Vang, E. Garza-Lopez, T. A. Christensen, J.-q. Shao, J. Lam, H. K. Beasley, A. G. Marshall, A. Crabtree, J. Anudokem, B. Rodriguez, B. Kirk, S. Bacevac, T. Barongan, , A. Hinton

This study uses 3D imaging to show that mitochondria in aging mouse hearts lose cristae structure and volume, similar to mutations in MICOS complex genes.

bioRxiv · Apr 23, 2022Biophysics

3D Reconstruction of Murine Mitochondria Exhibits Changes in Structure Across Aging Linked to the MICOS Complex

Z. Vue, E. Garza-Lopez, K. Neikirk, P. Katti, L. Vang, H. K. Beasley, J.-q. Shao, A. G. Marshall, A. Crabtree, A. C. Murphy, B. C. Jenkins, P. Prasad, C. S. Evans, B. T. Taylor, M. Mungai, , Murray

This study examines age-related changes in mitochondrial 3D structure in mouse muscle and heart tissues, linking these changes to the MICOS complex and metabolic alterations.

bioRxiv · Mar 22, 2022Biophysics