M. Mungai

This study identifies structural and metabolic changes in human heart failure using SBF-SEM and metabolomic analyses.

This study investigates age-related changes in mitochondrial 3D structure in human skeletal muscle and shows that exercise can restore mitochondrial integrity through mitofusin 2.

This study quantifies age-related changes in lipid droplet morphology and mitochondria-lipid interactions in murine brown adipose tissue using 3D imaging and analysis.

The paper describes a method to isolate skeletal muscle from mice, generate satellite cells for culture, and validate cell populations using immunofluorescence.

This paper describes a protocol for using proximity ligation assays (PLA) to study protein-protein interactions in mitochondria-endoplasmic reticulum contact sites (MERCs) in mouse skeletal muscle tissue and cells.

This paper presents an optimized western blot protocol to isolate and detect five OPA1 isoforms in muscle tissue.

This study shows that Sam50 deficiency in myotubes leads to mitochondrial fragmentation, altered metabolism, and impaired oxidative capacity in both mice and humans.

This study uses 3D electron microscopy to analyze mitochondrial cristae morphology in mouse brown adipose tissue across aging, finding significant structural changes in aged tissue.

This study shows that OPA1 deficiency in skeletal muscle increases MERC formation via ATF4, affecting calcium exchange and mitochondrial-ER tethering.

This study examines age-related changes in mitochondrial 3D structure in mouse muscle and heart tissues, linking these changes to the MICOS complex and metabolic alterations.

This paper describes methods to identify and distinguish subcellular structures involved in macroautophagy using TEM and 3D reconstruction, with applications to quantify degradation machinery under different conditions.

The paper introduces a standardized protocol using Amira software to quantify 3D organelle morphology from SBF-SEM data, demonstrating its application to mitochondria and ER structures.

This paper presents a method for preserving and quantitatively measuring mitochondrial morphology in tissues and cultured cells without introducing artifacts.

This paper introduces a standardized method using ImageJ to quantify subcellular organelle morphology in TEM images, focusing on mitochondria, ER, lysosomes, and autophagosomes.