S. A. Murray

Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation

The paper describes a method to isolate skeletal muscle from mice, generate satellite cells for culture, and validate cell populations using immunofluorescence.

Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells

This paper describes a protocol for using proximity ligation assays (PLA) to study protein-protein interactions in mitochondria-endoplasmic reticulum contact sites (MERCs) in mouse skeletal muscle tissue and cells.

Creating Optimal Conditions for OPA1 isoforms by Western Blot in Muscle Tissue

This paper presents an optimized western blot protocol to isolate and detect five OPA1 isoforms in muscle tissue.

Ablation of Sam50 is associated with fragmentation and alterations in metabolism in murine and human myotubes

This study shows that Sam50 deficiency in myotubes leads to mitochondrial fragmentation, altered metabolism, and impaired oxidative capacity in both mice and humans.

Focused ultrasound-mediated brain genome editing

This paper demonstrates a method to edit the brain genome in mammals using focused ultrasound to temporarily open the blood-brain barrier for CRISPR/Cas9 delivery via intravenous injection.

Three-Dimensional Mitochondria Reconstructions of Murine Cardiac Muscle Changes in Size Across Aging

This study uses 3D imaging to show that mitochondria in aging mouse hearts lose cristae structure and volume, similar to mutations in MICOS complex genes.

The Role of Mentoring in Promoting Diversity, Equity, and Inclusion in STEM Education and Research

The paper discusses the importance of mentoring in STEM for underrepresented minorities and presents workshop feedback showing student satisfaction despite low initial expectations.

An effective workshop on How to be an Effective Mentor for Underrepresented STEM Trainees

This workshop provides a framework for effective mentorship of underrepresented STEM trainees to address systemic issues in academic pipelines.

Using a Champion-Oriented Mindset to Overcome the Challenges of Graduate School

The paper describes a workshop aimed at preparing minority students for graduate school by fostering a champion-oriented mindset, showing it was well-received and perceived as helpful.

A comprehensive approach for artifact-free sample preparation and assessment of mitochondrial morphology in tissue and cultured cells

This paper presents a method for preserving and quantitatively measuring mitochondrial morphology in tissues and cultured cells without introducing artifacts.

Robust Mouse Tracking in Complex Environments using Neural Networks

This paper develops a neural network-based mouse tracker for complex environments, finding that an encoder-decoder segmentation model achieves high accuracy with minimal training data.