This study compares exosome and small ectosome secretion in HeLa cells using live tracking and proteomic analysis, revealing differences in their trafficking and release mechanisms.

bioRxiv · Oct 27, 2020Cell Biology

Molecular determinants of SR-B1-dependent Plasmodium sporozoite entry into hepatocytic cells.

A.-C. Langlois, G. Manzoni, L. Vincensini, R. Coppee, C. Marinach, M. Guerin, T. Huby, V. Carriere, F.-L. Cosset, M. Dreux, E. Rubinstein, O. Silvie

This study identifies structural determinants of SR-B1 required for Plasmodium sporozoite entry into hepatocytes using chimeric mouse/human SR-B1 constructs and structure-guided analysis.

bioRxiv · Mar 16, 2020Microbiology

Dynamic regulation of CD45 by tetraspanin CD53

V.-M. E. Dunlock, A. B. Arp, E. Jansen, S. Charrin, S. J. van Deventer, M. D. Wright, L. Querol-Cano, E. Rubinstein, A. B. van Spriel

This study identifies CD53 as a stabilizer of CD45RO, enhancing TCR signaling in T cells by maintaining CD45RO surface stability and function.

bioRxiv · Nov 25, 2019Immunology

Tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex

C. Z. Koo, N. Harrison, P. J. Noy, J. Szyroka, A. L. Matthews, H.-E. Hsia, S. A. Mueller, J. Tushaus, J. Goulding, K. Willis, C. Apicella, B. Cragoe, E. Davis, M. Keles, A. Malinova, , M. G. Tomlinson

The paper identifies Tspan15 as a critical partner of ADAM10, showing their interaction and functional synergy in proteolytic activity.

bioRxiv · Oct 11, 2019Cell Biology

HIV-1 specifically traps CD9 and CD81 tetraspanins within viral buds and induces their membrane depletion

S. Dahmane, C. Doucet, A. Le Gall, C. Chamontin, P. Dosset, F. Murcy, L. Fernandez, D. Salas Pastene, E. Rubinstein, M. Mougel, M. Nollmann, P.-E. Milhiet

This study shows HIV-1 traps CD9 and CD81 tetraspanins in viral buds, depleting them from cellular membranes and inhibiting virus-induced fusion.

bioRxiv · May 17, 2018Microbiology