F. Bottanelli

When less is more - A fast TurboID KI approach for high sensitivity endogenous interactome mapping

The paper compares labelling enzymes for proximity labelling experiments using CRISPR-Cas9 knock-ins, showing TurboID's effectiveness in identifying interactors and cargo proteins.

ARF1 compartments direct cargo flow via maturation into recycling endosomes

The paper identifies two functionally distinct ARF1 compartments involved in post-Golgi sorting and endocytic recycling, challenging the classical vesicle shuttling model.

Nanoscale imaging reveals the mechanisms of ER-to-Golgi transport via a dynamic tubular-vesicular network

This study uses live-cell imaging to show ER-to-Golgi transport occurs via a dynamic ARF4 tubular-vesicular network connected to the ER and Golgi.

Recombinant biosensors for multiplex and super-resolution imaging of phosphoinositides

This paper introduces recombinant biosensors with SNAP tags for multiplex and super-resolution imaging of all 8 phosphoinositides in fixed cells and tissues.

Sub-membrane actin rings compartmentalize the plasma membrane

This paper shows that sub-membrane actin rings compartmentalize the plasma membrane by confining membrane protein motion in a periodic pattern, observed in neurons and other cell types.

Gene editing and super-resolution microscopy reveal multiple distinct roles for ARF GTPases in cellular membrane organization

The paper uses gene editing and super-resolution microscopy to study the nanoscale localization of ARF GTPases, revealing distinct roles in membrane trafficking and compartment organization.

A versatile Halo- and SNAP-tagged BMP/TGFβ receptor library for quantification of cell surface ligand binding

The paper introduces a Halo- and SNAP-tagged BMP/TGFβ receptor library and establishes a Ligand Surface Binding Assay to quantify cell surface ligand binding specificity.

3D Adaptive Optical Nanoscopy for Thick Specimen Imaging at sub-50 nm Resolution

This paper develops a 3D adaptive optical nanoscopy technique that achieves sub-50 nm resolution in thick specimens by addressing optical aberrations.