J. E. Rothman

This paper introduces FLASH-PAINT, a method for rapid multiplexed super-resolution imaging of many molecular targets in mammalian cells, demonstrating its application in four cell biological studies.

This paper develops a platform for analyzing integral membrane proteins and lipids in intact membranes using native mass spectrometry, showing how lipid binding and membrane composition affect protein function.

The paper identifies molecular mechanisms by which Complexin clamps SNARE assembly in single-vesicle fusion, showing its role in delaying fusion and enhancing clamping through specific structural interactions.

This paper develops a 3D adaptive optical nanoscopy technique that achieves sub-50 nm resolution in thick specimens by addressing optical aberrations.

This study shows that Munc13 forms self-assembled clusters which are necessary for vesicle capture, with cluster size and the C2C domain affecting efficiency.

The paper shows TANGO1 membrane helices form a lipid diffusion barrier at curved membranes to prevent mixing during secretory cargo transport.

This paper introduces a super-resolution microscope that images whole mammalian cells at ~20 nm 3D resolution, revealing complex cellular structures previously only visible via electron microscopy.