J. Bewersdorf

This paper introduces FLASH-PAINT, a method for rapid multiplexed super-resolution imaging of many molecular targets in mammalian cells, demonstrating its application in four cell biological studies.

The paper shows Rtn4 oligomers increase ER membrane curvature by localizing to elliptical tubules and enhancing local density.

The paper introduces NanoWrap, a method for reconstructing subcellular membrane surfaces from single-molecule localization microscopy data, validated on simulated and experimental data.

This paper introduces a new microscopy technique called Unclearing Microscopy that allows visualization of subcellular structures with the unaided eye by expanding cells and opaquing them with light-scattering molecules.

This paper presents an integrated platform for high-throughput single-molecule localization microscopy (SMLM) that enables imaging of 10,000 cells per day.

The paper solves the native structure of a human GBP coatomer that coats cytosolic bacteria and functions as an antimicrobial signaling platform.

The paper introduces a new near-IR fluorophore that enables two-color live-cell SMLM without chemical additives or multiple lasers.

This study uses multi-omics profiling to analyze U2AF1 mutations in myeloid malignancies, revealing altered RNA binding, splicing, and stress granule formation.

This paper develops a 3D adaptive optical nanoscopy technique that achieves sub-50 nm resolution in thick specimens by addressing optical aberrations.

The paper shows that DMA-tudor interactions drive the formation of specific membraneless organelles by controlling condensate assembly and composition.

This paper introduces a method to expand cellular structures while preserving proteins, enabling efficient labeling and visualization of protein distributions at the nanoscale using standard light microscopy.

This study demonstrates that customized speckle patterns can achieve three times higher spatial resolution than the diffraction limit in parallelized nonlinear pattern-illumination microscopy.

This study investigates how formaldehyde fixation and imaging conditions affect the photophysics of mEos3.2 in fission yeast cells, providing a guide for quantitative imaging and counting of mEos3.2-tagged molecules.

This paper introduces a fluorogenic DNA-PAINT probe that reduces background and increases imaging speed for 3D whole-cell DNA-PAINT.

The paper develops a method to improve 4Pi single-molecule localization microscopy by fitting an experimental PSF model, achieving the theoretical resolution limit.

This paper develops a STED system for 3D super-resolution imaging in living mice, achieving depths of 76 micrometers in brain tissue.

This paper introduces a super-resolution microscope that images whole mammalian cells at ~20 nm 3D resolution, revealing complex cellular structures previously only visible via electron microscopy.

This study identifies EFCAB9 as a pH-dependent Ca2+ sensor that modulates CatSper channel activity and sperm motility through its interaction with CATSPERζ.

The paper introduces NEP fitting to measure resolution and cellular features in live-cell STED images without beads or pre-calibration, validated on microtubules and applied to ER tubules.

The paper uses microscopy techniques to study interphase nodes in fission yeast, finding they have defined sizes and protein compositions, with type 1 nodes disassembling during mitosis and type 2 nodes remaining intact.