J. Broichhagen

This study shows that GLP-1 receptor activation in the olfactory bulb enhances insulin secretion via a sympathetic pathway involving the paraventricular nucleus.

This paper demonstrates that MINFLUX fluorescence nanoscopy can measure intra-molecular distances down to 1 Angstrom with high precision, validated using known protein distances and subunit orientations.

This study compares the trafficking and signaling of GLP-1R and GIPR in pancreatic beta cells, revealing differences in internalization, recycling, and subcellular activity.

This study shows that 14-3-3ζ regulates insulin secretion in pancreatic β-cells by enhancing mitochondrial function and ATP synthesis, with implications for diabetes.

The paper introduces a Halo- and SNAP-tagged BMP/TGFβ receptor library and establishes a Ligand Surface Binding Assay to quantify cell surface ligand binding specificity.

This study develops a transgenic pig model with SNAP-tagged insulin to investigate in vivo insulin secretory granule dynamics and turnover.

This study compares the efficacy and affinity of two GLP-1R agonists, P5 and exendin-F1, showing that exendin-F1's lower acute efficacy correlates with sustained insulin secretion and anti-hyperglycaemic effects.

This study compares exendin-4 and its acylated variant exendin-4-C16, showing acylation affects GLP-1R signaling bias, trafficking, and pharmacokinetics.

The paper introduces sulfonated rhodamines as impermeable dyes for cell surface protein labeling, validated in live cell imaging and STED nanoscopy.

The paper investigates the dimerization behavior of F220C rhodopsin mutant and wild-type opsin in vitro and in the plasma membrane, finding that the mutant forms a unique dimer with distinct conformational features.

This paper introduces deuterated rhodamines for improved fluorescence microscopy applications, showing enhanced photophysical and chemical properties.

The paper purifies age-distinct insulin secretory granules from INS-1 cells and identifies age-related changes in their lipid composition and membrane properties.

The paper evaluates how peptide context affects GLP-1R signaling and trafficking, showing differences in agonist-mediated responses based on sequence homology and structural features.

The paper introduces SBG-conjugated fluorophores to improve extracellular labeling of SNAP-fused proteins, enabling precise analysis of GPCR populations in living cells and tissues.

This study compares the signalling, trafficking, and glucoregulatory effects of exendin-4 and lixisenatide, finding differences in cAMP signalling potency and receptor recycling that impact glucose control.

The paper introduces LUXendin peptides that label and visualize endogenous GLP1R with high specificity, revealing receptor organization and dynamics.

This paper develops branched photoswitchable ligands for efficient optical control and detection of class C G protein-coupled receptors, demonstrating near-complete agonism and dual imaging/manipulation capabilities.

This paper introduces a method to reversibly control GPCR activity using photoswitchable ligands tethered via SNAP-tagged nanobodies.