G. Knott

Obtaining high-resolution cryo-EM structures using a common LaB6, 120-keV electron microscope equipped with a sub 200-keV optimised direct electron detector.

This paper demonstrates that a standard 120-keV TEM with a direct electron detector can achieve high-resolution cryo-EM reconstructions of various proteins.

Mechanisms of axoneme and centriole elimination in Naegleria gruberi

This study identifies mechanisms by which Naegleria gruberi eliminates axonemes and centrioles during life cycle reversion, revealing lysosomal and proteasome-dependent pathways and extracellular shedding.

A sexually dimorphic hepatic cycle of periportal VLDL generation and subsequent pericentral VLDLR-mediated lipoprotein re-uptake

The paper identifies a sexually dimorphic hepatic cycle of VLDL generation and re-uptake in mice and humans, linking VLDLR expression to cardiovascular risk.

Early invasion of uropathogenic Escherichia coli into the bladder wall by solitary bacteria that are protected from antibiotics and neutrophil swarms in an organoid model

This study uses a bladder organoid model to show that UPEC can invade deeper bladder layers as solitary bacteria, evading antibiotics and neutrophils, forming reservoirs that contribute to recurrent infections.

Molecular insights into Vibrio cholerae’s intra-amoebal host-pathogen interactions

This study identifies molecular mechanisms enabling Vibrio cholerae to replicate within amoebae and escape, highlighting the role of minor virulence factors in its aquatic survival.