K. Sharma

The sensory shark: high-quality morphological, genomic and transcriptomic data for the small spotted catshark Scyliorhinus canicula reveal the molecular bases of sensory organ evolution in jawed vertebrates

This study integrates genomic, transcriptomic, and morphological data from the small-spotted catshark to uncover molecular mechanisms underlying sensory organ evolution in jawed vertebrates.

HIV-1 infection is associated with depletion of germinal center B cells and a decrease in IgA+ plasma cells in the GI tract

Translating Ethiopian potato seed networks: Identifying strategic intervention points for managing bacterial wilt and other diseases

This study identifies key locations and strategies to manage bacterial wilt spread in Ethiopia's potato seed systems through network analysis and risk assessment.

P66Shc Mediates SUMO2-induced Endothelial Dysfunction

This study identifies SUMO2-mediated modification of p66Shc as a mechanism causing endothelial dysfunction and oxidative stress in hyperlipidemia.

Extreme in Every Way: Exceedingly Low Genetic Diversity in Snow Leopards Due to Persistently Small Population Size

This study uses whole-genome sequencing of 41 snow leopards to show they have the lowest genetic diversity of any big cat species due to long-term small population size, not recent inbreeding.

Lack of Purple Acid Phosphatase SlPAP26b compromises the phosphorous starvation response in tomato independent of SlPHR1 and SlPHL1

This study identifies SlPAP26b as a key player in tomato's phosphorus starvation response, independent of SlPHR1 and SlPHL1, through transcriptomics and proteomics analysis.

Essential oil terpenes may inhibit steroidogenic cytochrome P450 activities.

This study investigates the effects of essential oil components on steroidogenic enzymes CYP17A1 and CYP19A1, finding some compounds inhibit androgen production and may impact prostate cancer and PCOS.

Accelerated design of Escherichia coli genomes with reduced size using a whole-cell model and machine learning

The paper uses a whole-cell model and machine learning to design a reduced E. coli genome with 40% fewer genes, achieving 95% faster computation.

Bacterial pore-forming toxin pneumolysin drives pathogenicity through shed toxin-loaded host extracellular vesicles

This study shows that pneumolysin-loaded host extracellular vesicles contribute to pneumococcal pathogenesis by inducing cytotoxicity and inflammation.

Kinase inhibitor-induced cell-type specific vacuole formation in the absence of canonical ATG5-dependent autophagy

This study shows that SB202190 induces cell-type specific vacuole formation independent of canonical autophagy, involving RAB7 and revealing potential therapeutic strategies.

Single-cell transcriptomic and TCR analysis of human Cytomegalovirus (hCMV)-specific memory T cells reveals effector and pre-effectors of CD8+- and CD4+-cytotoxic T cells

The paper characterizes hCMV-specific memory T cells using single-cell transcriptomics and TCR analysis, identifying distinct functional subsets and their roles in immunity.

Introgression of a dominant phototropin1 mutant superenhances carotenoids and boosts flavor-related volatiles in genome-edited tomato RIN mutants

This study introduces a tomato cultivar with extended shelf life, improved taste, and flavor by combining genome-edited ripening mutants with a phototropin1 mutant.

Acetyl transferase EP300 deficiency leads to chronic replication stress mediated by defective fork protection at stalled replication forks

This paper shows that EP300 deficiency causes chronic replication stress and genomic instability in ATLL cells by impairing fork protection and leading to DNA damage and mitotic defects.

Gut-associated lymphoid tissue attrition associates with response to anti-α4β7 therapy in ulcerative colitis

This study investigates the mechanism of vedolizumab in ulcerative colitis by analyzing immune cell changes in gut-associated lymphoid tissue and shows that targeting GALT is a novel mechanism of action.

Ancient and nonuniform loss of olfactory receptor expression renders the shark nose a de facto vomeronasal organ

The paper investigates the evolutionary dynamics of olfactory receptor families in cartilaginous fishes, finding that V2R/OlfC receptors are expressed in microvillous neurons, while other families are underrepresented.

Profiling of the β-glucosidases identified in the genome of Penicillium funiculosum: Insights from genomics, transcriptomics, proteomics and homology modelling studies

This study profiles β-glucosidases in Penicillium funiculosum using genomics, transcriptomics, proteomics, and molecular dynamics, identifies key enzymes, and improves thermostability through protein engineering.

Empagliflozin reduces renal lipotoxicity in experimental Alport syndrome

This study shows empagliflozin reduces renal lipotoxicity and improves kidney function in experimental Alport syndrome by shifting energy substrates from glucose to fatty acids in podocytes.

Sustained pigmentation causes DNA damage and invokes translesion polymerase Pol κ for repair in melanocytes

This study shows sustained melanogenesis causes DNA damage in melanocytes, invokes Pol κ for repair, and links Pol κ activity to genome instability and mutational load in melanoma.

Consideration of genetic variation and evolutionary history in future conservation of Indian one-horned rhinoceros (Rhinoceros unicornis)

This study sequenced the mitogenome of Indian one-horned rhinos to assess genetic variation and evolutionary history, identifying three distinct genetic clades and proposing three Evolutionary Significant Units for conservation.

Obesity impairs therapeutic efficacy of mesenchymal stem cells by inhibiting cardiolipin-dependent mitophagy and intercellular mitochondrial transfer in mouse models of airway allergic inflammation

This study shows that obesity impairs MSCs' ability to perform mitophagy and intercellular mitochondrial transfer, and that PQQ can restore these functions in obese-derived MSCs.

A novel innate pathogen sensing strategy involving ubiquitination of bacterial surface proteins

The paper identifies a novel innate pathogen sensing strategy through ubiquitination of bacterial surface proteins, revealing a conserved mechanism for pathogen detection and clearance.

Allosteric cooperation in β-lactam binding to a non-classical transpeptidase

The paper identifies an allosteric site in a L,D-transpeptidase that binds the sugar moiety of PG substrates and a second β-lactam molecule, revealing cooperative binding with the catalytic site.

PDE9 Inhibition Activates PPARa to Stimulate Mitochondrial Fat Metabolism and Reduce Cardiometabolic Syndrome

This study shows PDE9 inhibition activates PPARa to enhance mitochondrial fat metabolism and reduce cardiometabolic syndrome in mice, with potential for treating obesity in men and postmenopausal women.

Dynamic persistence of intracellular bacterial communities of uropathogenic Escherichia coli in a human bladder-chip model of urinary tract infections

This study uses a bladder-chip model to show that intracellular bacterial communities in UPEC persist despite antibiotic treatment and recovery periods, leading to reinfection.

Scalable production of tissue-like vascularised liver organoids from human PSCs.

This paper develops a method to produce vascularised liver organoids from human PSCs without 2D patterning or ECM, demonstrating liver-like functions and cellular complexity.

A lung-on-chip model reveals an essential role for alveolar epithelial cells in controlling bacterial growth in early tuberculosis

This study uses a lung-on-chip model to show that alveolar epithelial cell-derived surfactant limits early Mycobacterium tuberculosis growth by removing virulence factors.

Early invasion of uropathogenic Escherichia coli into the bladder wall by solitary bacteria that are protected from antibiotics and neutrophil swarms in an organoid model

This study uses a bladder organoid model to show that UPEC can invade deeper bladder layers as solitary bacteria, evading antibiotics and neutrophils, forming reservoirs that contribute to recurrent infections.

The new kid on the block: A dominant-negative mutation of phototropin1 enhances carotenoid content in tomato fruits

A dominant-negative mutation in phototropin1 increases carotenoid content and alters metabolite and protein profiles in tomato fruits.

Spatial variation in population-density of snow leopards in a multiple use landscape in Spiti Valley, Trans-Himalaya

This study estimates snow leopard density in Spiti Valley using camera trap data and identifies factors influencing their distribution.

A Multimodal and Integrated Approach to Interrogate Human Kidney Biopsies with Rigor and Reproducibility: The Kidney Precision Medicine Project

The paper describes a pipeline for generating 3D molecular atlases of human kidney biopsies using multi-OMICS and imaging technologies, emphasizing quality control and reproducibility across institutions.