K. Luger

A FACT-ETS-1 Antiviral Response Pathway Restricts Viral Replication and is Countered by Poxvirus A51R Proteins

This study identifies a FACT-ETS-1 antiviral pathway that restricts poxvirus replication and shows how viral A51R proteins counteract it.

Dynamics of endogenous PARP1 and PARP2 during DNA damage revealed by live-cell single-molecule imaging

This study uses live-cell single-molecule imaging to show that most PARP1/2 diffuse freely, with a small fraction transiently binding DNA after damage, suggesting new strategies for PARP inhibitors.

Structure and function of the chromatin remodeler SMARCAD1 with its nucleosome substrate

This study reveals the structure and mechanism of SMARCAD1, showing it transfers histone octamers between DNA segments and can assemble de novo nucleosomes.

Automated Modeling of Protein Accumulation at DNA Damage Sites using qFADD.py

The paper introduces qFADD.py, an open-source tool for analyzing DNA damage repair protein dynamics using automated fitting and simulation, improving upon previous methods with better statistical rigor and user-friendliness.

HPF1 and nucleosomes mediate a dramatic switch in activity of PARP1 from polymerase to hydrolase

This study investigates how HPF1 modulates PARP1 activity, switching it from polymerase to hydrolase, and shows HPF1's role in redirecting PARylation and affecting PAR chain length.

Archaeal chromatin 'slinkies' are inherently dynamic complexes with deflected DNA wrapping pathways

This study characterizes the dynamic structure of archaeal chromatin complexes using simulations and cryoEM, revealing their inherent flexibility and DNA wrapping patterns.

Bridging of nucleosome-proximal DNA double-strand breaks by PARP2 enhances its interaction with HPF1

The paper presents a cryo-EM structure of PARP2 bridging nucleosome-proximal DNA breaks and shows its interaction with HPF1 in a functional complex.

Q-FADD: A mechanistic approach for modeling the accumulation of proteins at sites of DNA damage by free diffusion

The paper introduces a free diffusion model to quantify protein accumulation at DNA damage sites in live cells, applied to poly-ADP-ribose polymerases 1 and 2.

Poly(ADP-ribose) polymerase 1 Searches DNA via a ‘Monkey Bar’ Mechanism

This study reveals that PARP1 searches DNA using a 'monkey bar' mechanism, where DNA binding facilitates release, and identifies the WGR-domain as essential for this process.

The histone chaperone FACT modulates nucleosome structure by tethering its components

This study shows that FACT facilitates nucleosome formation by tethering H2A-H2B and promoting hexasome and nucleosome assembly.