J. Rudolph

A nonenzymatic dependency on inositol-requiring enzyme 1 controls cancer cell cycle progression and tumor growth

This study identifies a nonenzymatic role of IRE1 in cancer cell cycle progression and tumor growth, independent of its kinase-RNase activity.

Targeting the Hippo pathway in cancers via ubiquitination dependent TEAD degradation

This study identifies RNF146 as an E3 ubiquitin ligase that degrades TEAD proteins via the proteasome, showing that pharmacological induction of TEAD ubiquitination can suppress YAP/TAZ-driven cancer growth.

Automated Modeling of Protein Accumulation at DNA Damage Sites using qFADD.py

The paper introduces qFADD.py, an open-source tool for analyzing DNA damage repair protein dynamics using automated fitting and simulation, improving upon previous methods with better statistical rigor and user-friendliness.

The structure of the human LACTB filament reveals the mechanisms of assembly and membrane binding

This study determines the structure of human LACTB filaments, revealing their assembly mechanism and membrane-binding properties.

Dynamics of endogenous PARP1 and PARP2 during DNA damage revealed by live-cell single-molecule imaging

This study uses live-cell single-molecule imaging to show that most PARP1/2 diffuse freely, with a small fraction transiently binding DNA after damage, suggesting new strategies for PARP inhibitors.

Noncanonical mRNA decay by the endoplasmic-reticulum stress sensor IRE1α promotes cancer-cell survival

The paper identifies a noncanonical mRNA decay process, RIDDLE, mediated by IRE1α oligomers in cancer cells, which promotes survival by degrading mRNAs without XBP1-like endomotifs.

HPF1 and nucleosomes mediate a dramatic switch in activity of PARP1 from polymerase to hydrolase

This study investigates how HPF1 modulates PARP1 activity, switching it from polymerase to hydrolase, and shows HPF1's role in redirecting PARylation and affecting PAR chain length.

Bridging of nucleosome-proximal DNA double-strand breaks by PARP2 enhances its interaction with HPF1

The paper presents a cryo-EM structure of PARP2 bridging nucleosome-proximal DNA breaks and shows its interaction with HPF1 in a functional complex.

Activation loop dynamics are controlled by conformation-selective inhibitors of ERK2

This paper shows how different ERK2 inhibitors shift conformational states by exploiting activation loop dynamics, affecting dephosphorylation rates.

Disruption of IRE1α through its Kinase Domain Attenuates Multiple Myeloma

This study shows that disrupting IRE1α's kinase domain reduces multiple myeloma tumor growth and enhances treatment efficacy, suggesting it as a therapeutic target.

Q-FADD: A mechanistic approach for modeling the accumulation of proteins at sites of DNA damage by free diffusion

The paper introduces a free diffusion model to quantify protein accumulation at DNA damage sites in live cells, applied to poly-ADP-ribose polymerases 1 and 2.

Poly(ADP-ribose) polymerase 1 Searches DNA via a ‘Monkey Bar’ Mechanism

This study reveals that PARP1 searches DNA using a 'monkey bar' mechanism, where DNA binding facilitates release, and identifies the WGR-domain as essential for this process.