Author
Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.
5 papers
Recent·Most cited
D. A. Bonsor, P. Alexander, K. Snead, N. Hartig, M. Drew, S. Messing, L. I. Finci, D. V. Nissley, F. McCormick, D. Esposito, P. Rodriguez-Viciana, A. G. Stephen, D. K. Simanshu
The paper presents the structure of the SHOC2-MRAS-PP1C complex and shows how it regulates RAF activation and is linked to Noonan syndrome mutations.
bioRxiv · May 10, 2022Biophysics
K. Snead, V. Wall, H. Ambrose, D. Esposito, M. Drew
This paper demonstrates that using SUGT1 chaperone with polycistronic baculovirus expression improves yield and quality of leucine-rich repeat proteins and complexes.
bioRxiv · Jan 19, 2022Biochemistry
J. Mehalko, M. Drew, K. Snead, J.-P. Denson, V. Wall, T. Taylor, K. Sadtler, S. Messing, W. Gillette, D. Esposito
The paper improves the production of SARS-CoV-2 spike RBD for serology assays by optimizing DNA constructs, purification parameters, and demonstrating the role of a carboxyterminal tag in sensitivity.
bioRxiv · Nov 18, 2020Biochemistry
D. Esposito, J. Mehalko, M. Drew, K. Snead, V. Wall, T. Taylor, P. Frank, J.-P. Denson, M. Hong, G. Gulten, K. Sadtler, S. Messing, W. Gillette
This study improves the yield of SARS-CoV-2 spike protein for serology assays by optimizing expression and purification parameters, achieving 5 mg/L production.
bioRxiv · May 27, 2020Biochemistry
M. Sherekar, S.-W. Han, R. Ghirlando, S. Messing, M. Drew, P. Juneja, H. O'Neill, C. Stanley, D. Bhowmik, A. Ramanathan, S. Subramaniam, D. V. Nissley, W. Gillette, F. McCormick, D. Esposito
This study shows Neurofibromin forms a high-affinity dimer through biochemical and structural analysis, with implications for its function in RAS-MAPK signaling.
bioRxiv · Sep 5, 2019 · PreprintBiochemistry