Author

D. Esposito

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Biochemistry· 4 Biophysics· 3 Cancer Biology· 2 Immunology· 1 Pharmacology & Toxicology· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:experimental assay· 10 Organism:human· 5 Assay:proteomics· 5 Research Focus:cell type annotation· 3 Research Focus:drug response / drug discovery· 3 Research Focus:host-pathogen & immune response· 3 Research Focus:protein folding & dynamics· 3 Approach:biophysical modeling· 2

Frequent Co-Authors

M. Drew· 5 shared S. Messing· 5 shared K. Snead· 4 shared D. V. Nissley· 3 shared F. McCormick· 3 shared J.-P. Denson· 3 shared T. Taylor· 3 shared V. Wall· 3 shared

Activity by Year

2019-2023 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 41 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

5 shared papersBiochemistry

M. Drew

Mutual co-authors in cluster

Author profile →

11 papers

Recent·Most cited

Assembly reactions of SARS-CoV-2 nucleocapsid protein with nucleic acid

H. Zhao, A. M. Syed, M. M. Khalid, A. Nguyen, A. Ciling, D. Wu, W.-M. Yau, S. Srinivasan, D. Esposito, J. A. Doudna, G. Piszczek, M. Ott, P. Schuck

This study investigates the assembly of SARS-CoV-2 nucleocapsid protein with nucleic acids using biophysical techniques and mutant proteins, revealing key interaction motifs and stabilization mechanisms.

bioRxiv · Nov 22, 2023 · PreprintBiophysics

The Multidrug Resistance Transporter P-glycoprotein Confers Resistance to Ferroptosis Inducers

W. J. E. Frye, L. M. Huff, J. M. G. Dalmasy, P. Salazar, R. M. Carter, R. T. Gensler, D. Esposito, R. W. Robey, S. V. Ambudkar, M. M. Gottesman

This study shows that P-glycoprotein (P-gp) and ABCG2 confer resistance to ferroptosis inducers, and some inducers inhibit these transporters, suggesting potential drug-drug interactions.

bioRxiv · Feb 23, 2023Pharmacology & Toxicology

Structure of the SHOC2-MRAS-PP1C complex provides insights into RAF activation and Noonan syndrome

D. A. Bonsor, P. Alexander, K. Snead, N. Hartig, M. Drew, S. Messing, L. I. Finci, D. V. Nissley, F. McCormick, D. Esposito, P. Rodriguez-Viciana, A. G. Stephen, D. K. Simanshu

The paper presents the structure of the SHOC2-MRAS-PP1C complex and shows how it regulates RAF activation and is linked to Noonan syndrome mutations.

bioRxiv · May 10, 2022Biophysics

CK2 signaling from TOLLIP-dependent perinuclear endosomes is an essential feature of KRAS and NRAS mutant cancers

S. Basu, B. Luke, B. Karim, N. Martin, S. Lockett, S. Das, T. Andresson, K. Saylor, S. Kozlov, L. Bassel, D. Esposito, M. Galloux, P. F. Johnson

This study identifies TOLLIP as a critical adaptor for CK2 signaling from perinuclear endosomes in KRAS and NRAS mutant cancers, showing its role in tumor proliferation and progression.

bioRxiv · Apr 5, 2022 · PreprintCancer Biology

Polycistronic baculovirus expression of SUGT1 enables high-yield production of recombinantleucine-rich repeat proteins and protein complexes

K. Snead, V. Wall, H. Ambrose, D. Esposito, M. Drew

This paper demonstrates that using SUGT1 chaperone with polycistronic baculovirus expression improves yield and quality of leucine-rich repeat proteins and complexes.

bioRxiv · Jan 19, 2022Biochemistry

Camel nanobodies broadly neutralize SARS-CoV-2 variants

J. Hong, H. J. Kwon, R. Cachau, C. Z. Chen, K. J. Butay, Z. Duan, D. Li, H. Ren, T. Liang, J. Zhu, V. P. Dandey, N. P. Martin, D. Esposito, U. Ortega-Rodriguez, M. Xu, , M. Ho

The paper isolates two camel nanobodies that broadly neutralize SARS-CoV-2 and its variants, showing therapeutic potential in mice.

bioRxiv · Oct 27, 2021Immunology

Improved production of SARS-CoV-2 spike receptor-binding domain (RBD) for serology assays

J. Mehalko, M. Drew, K. Snead, J.-P. Denson, V. Wall, T. Taylor, K. Sadtler, S. Messing, W. Gillette, D. Esposito

The paper improves the production of SARS-CoV-2 spike RBD for serology assays by optimizing DNA constructs, purification parameters, and demonstrating the role of a carboxyterminal tag in sensitivity.

bioRxiv · Nov 18, 2020Biochemistry

Live imaging of breast tumors shows macrophage-dependent induction and TMEM-mediated enrichment of cancer stem cells during metastatic dissemination

V. P. Sharma, B. Tang, Y. Wang, G. S. Karagiannis, E. A. Xue, D. Entenberg, L. Borriello, A. Coste, C. L. Duran, R. J. Eddy, G. Kim, X. Ye, J. G. Jones, E. Grunblatt, N. Agi, , M. H. Oktay

The paper uses intravital imaging to show macrophage-induced cancer stem cells (CSCs) are enriched at TMEM doorways during metastasis, with CSCs comprising >60% of circulating tumor cells.

bioRxiv · Sep 18, 2020 · PreprintCancer Biology

KRAS interaction with RAF1 RAS-binding domain and cysteine-rich domain provides insights into RAS-mediated RAF activation

T. H. Tran, A. H. Chan, L. C. Young, L. Bindu, C. Neale, S. Messing, S. Dharmaiah, T. Taylor, J.-P. Denson, D. Esposito, D. V. Nissley, A. G. Stephen, F. McCormick, D. K. Simanshu

The paper determines crystal structures of KRAS mutants bound to RAF1 domains, revealing molecular interactions critical for RAS-mediated RAF activation.

bioRxiv · Jul 31, 2020Biophysics

Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays

D. Esposito, J. Mehalko, M. Drew, K. Snead, V. Wall, T. Taylor, P. Frank, J.-P. Denson, M. Hong, G. Gulten, K. Sadtler, S. Messing, W. Gillette

This study improves the yield of SARS-CoV-2 spike protein for serology assays by optimizing expression and purification parameters, achieving 5 mg/L production.

bioRxiv · May 27, 2020Biochemistry

Biochemical and structural analysis reveals the Neurofibromin (NF1) protein forms a high-affinity dimer

M. Sherekar, S.-W. Han, R. Ghirlando, S. Messing, M. Drew, P. Juneja, H. O'Neill, C. Stanley, D. Bhowmik, A. Ramanathan, S. Subramaniam, D. V. Nissley, W. Gillette, F. McCormick, D. Esposito

This study shows Neurofibromin forms a high-affinity dimer through biochemical and structural analysis, with implications for its function in RAS-MAPK signaling.

bioRxiv · Sep 5, 2019 · PreprintBiochemistry