Author

S. Messing

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 4 Biophysics· 2

Signature Assays, Organisms & Methods

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Approach:experimental assay· 6 Research Focus:drug response / drug discovery· 3 Assay:proteomics· 3 Approach:biophysical modeling· 2 Organism:human· 2 Research Focus:protein folding & dynamics· 2 Assay:structural biology· 2 Research Focus:cell signaling & trafficking· 1

Frequent Co-Authors

D. Esposito· 5 shared F. McCormick· 4 shared M. Drew· 4 shared A. G. Stephen· 3 shared D. K. Simanshu· 3 shared D. V. Nissley· 3 shared J.-P. Denson· 3 shared K. Snead· 3 shared

Activity by Year

2019-2022 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 43 mutual co-author links

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5 shared papersBiochemistry

D. Esposito

Mutual co-authors in cluster

Author profile →

6 papers

Recent·Most cited

Ras-dependent RAF-MAPK hyperactivation by pathogenic RIT1 is a therapeutic target in Noonan syndrome-associated cardiac hypertrophy

A. Cuevas-Navarro, M. Wagner, R. Van, M. Swain, M. R. Allison, A. Cheng, S. Messing, D. K. Simanshu, M. J. Sale, F. McCormick, A. G. Stephen, P. Castel

This study identifies how pathogenic RIT1 mutations drive RAF-MAPK hyperactivation in Noonan syndrome, showing MEK inhibition can alleviate cardiac hypertrophy in a mouse model.

bioRxiv · Nov 2, 2022Biochemistry

Structure of the SHOC2-MRAS-PP1C complex provides insights into RAF activation and Noonan syndrome

D. A. Bonsor, P. Alexander, K. Snead, N. Hartig, M. Drew, S. Messing, L. I. Finci, D. V. Nissley, F. McCormick, D. Esposito, P. Rodriguez-Viciana, A. G. Stephen, D. K. Simanshu

The paper presents the structure of the SHOC2-MRAS-PP1C complex and shows how it regulates RAF activation and is linked to Noonan syndrome mutations.

bioRxiv · May 10, 2022Biophysics

Improved production of SARS-CoV-2 spike receptor-binding domain (RBD) for serology assays

J. Mehalko, M. Drew, K. Snead, J.-P. Denson, V. Wall, T. Taylor, K. Sadtler, S. Messing, W. Gillette, D. Esposito

The paper improves the production of SARS-CoV-2 spike RBD for serology assays by optimizing DNA constructs, purification parameters, and demonstrating the role of a carboxyterminal tag in sensitivity.

bioRxiv · Nov 18, 2020Biochemistry

KRAS interaction with RAF1 RAS-binding domain and cysteine-rich domain provides insights into RAS-mediated RAF activation

T. H. Tran, A. H. Chan, L. C. Young, L. Bindu, C. Neale, S. Messing, S. Dharmaiah, T. Taylor, J.-P. Denson, D. Esposito, D. V. Nissley, A. G. Stephen, F. McCormick, D. K. Simanshu

The paper determines crystal structures of KRAS mutants bound to RAF1 domains, revealing molecular interactions critical for RAS-mediated RAF activation.

bioRxiv · Jul 31, 2020Biophysics

Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays

D. Esposito, J. Mehalko, M. Drew, K. Snead, V. Wall, T. Taylor, P. Frank, J.-P. Denson, M. Hong, G. Gulten, K. Sadtler, S. Messing, W. Gillette

This study improves the yield of SARS-CoV-2 spike protein for serology assays by optimizing expression and purification parameters, achieving 5 mg/L production.

bioRxiv · May 27, 2020Biochemistry

Biochemical and structural analysis reveals the Neurofibromin (NF1) protein forms a high-affinity dimer

M. Sherekar, S.-W. Han, R. Ghirlando, S. Messing, M. Drew, P. Juneja, H. O'Neill, C. Stanley, D. Bhowmik, A. Ramanathan, S. Subramaniam, D. V. Nissley, W. Gillette, F. McCormick, D. Esposito

This study shows Neurofibromin forms a high-affinity dimer through biochemical and structural analysis, with implications for its function in RAS-MAPK signaling.

bioRxiv · Sep 5, 2019 · PreprintBiochemistry