O. Beckstein

Kinetic Diagram Analysis: A Python Library for Calculating Steady-State Observables of Biochemical Systems Analytically

KDA is a Python library that generates symbolic expressions for steady-state observables of biochemical systems, demonstrating its use on transporters with varying states and kinetic rates.

Chloride binding to prestin does not influence very high-frequency complex nonlinear capacitance (cNLC) in the mouse outer hair cell

This study shows chloride binding does not affect very high-frequency nonlinear capacitance in mouse outer hair cells, suggesting mechanical loads, not anion interactions, govern high-frequency prestin function.

Mechanism of substrate binding and transport in BASS transporters

This study determines the crystal structure of a BASS transporter in complex with pantoate, revealing how substrate binding is coupled to sodium ion binding and transport through conformational changes.

Thermodynamically consistent determination of free energies and rates in kinetic cycle models

This paper introduces a maximum likelihood method called multibind to create thermodynamically consistent models from kinetic and thermodynamic measurements.

Energy Coupling and Stoichiometry of Zn2+/H+ Antiport by the Cation Diffusion Facilitator YiiP

This study investigates the structural and functional roles of Zn2+/H+ antiport in YiiP using cryo-EM, thermophoresis, and simulations, revealing a transport stoichiometry of 1 Zn2+ to 2-3 H+.

Structure and lipid-mediated remodelling mechanism of the Na+/H+ exchanger NHA2

This paper reports the cryo-EM structures of NHA2 and reveals its lipid-mediated dimerization mechanism for ion exchange.

Zinc Dependent Conformational Changes in the Cation Diffusion Facilitator YiiP from S. oneidensis

This study examines how Zn2+ binding affects the conformational changes in YiiP from S. oneidensis using cryo-EM and molecular dynamics simulations, revealing an intermediate state and a hydrophobic gate closure mechanism.