Author

S. Darst

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 3

Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Organism:bacteria (other)· 2 Assay:cryo-EM· 2 Research Focus:chromatin & epigenetic regulation· 1 Assay:structural biology· 1

Frequent Co-Authors

J. Chen· 2 shared J. Y. Kang· 2 shared A. J. Noble· 1 shared B. Carragher· 1 shared B. Malone· 1 shared C. Fan· 1 shared C. M. Nimigean· 1 shared C. S. Potter· 1 shared

Activity by Year

2018-2020 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 31 mutual co-author links

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2 shared papersBiophysics

J. Chen

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3 papers

Recent·Most cited

Time-resolved cryoEM using Spotiton

V. P. Dandey, W. C. Budell, H. Wei, D. Bobe, K. Maruthi, M. Kopylov, E. T. Eng, P. A. Kahn, J. E. Hinshaw, N. Kundu, C. M. Nimigean, C. Fan, N. Sukomon, S. Darst, R. Saecker, , B. Carragher

The paper introduces a method for preparing cryoEM grids to study short-lived molecular states by rapidly dispensing sample drops and vitrifying them.

Microscopy and Microanalysis · Jul 30, 2020Peer-reviewedBiophysics

Eliminating effects of particle adsorption to the air/water interface in single-particle cryo-electron microscopy: Bacterial RNA polymerase and CHAPSO

J. Chen, A. J. Noble, J. Y. Kang, S. Darst

This study shows that CHAPSO detergent reduces orientation bias in bacterial transcription complexes by preventing particle adsorption at the air/water interface during cryo-EM.

bioRxiv · Oct 30, 2018Biophysics

Structural basis for transcript elongation control by NusG/RfaH universal regulators

J. Y. Kang, R. A. Mooney, Y. Nedialkov, J. Saba, T. V. Mishanina, I. Artsimovitch, R. Landick, S. Darst

This study determines the structural basis of how NusG and RfaH regulate transcript elongation by suppressing backtracking and pausing in bacterial RNA polymerase complexes.

bioRxiv · May 16, 2018Biophysics