Author

B. Malone

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 5 Biochemistry· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 5 Assay:cryo-EM· 4 Approach:microscopy & imaging analysis· 3 Organism:virus· 3 Organism:E. coli· 2 Assay:structural biology· 2 Research Focus:chromatin & epigenetic regulation· 1 Research Focus:host-pathogen & immune response· 1

Frequent Co-Authors

E. T. Eng· 6 shared J. Chen· 6 shared S. A. Darst· 5 shared K. Maruthi· 4 shared E. A. Campbell· 3 shared E. Llewellyn· 3 shared B. Carragher· 2 shared C. S. Potter· 2 shared

Activity by Year

2020-2024 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 36 mutual co-author links

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6 shared papersBiophysics

E. T. Eng

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Author profile →

6 papers

Recent·Most cited

Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy

R. M. Saecker, A. U. Mueller, B. Malone, J. Chen, W. C. Budell, V. P. Dandey, K. Maruthi, J. H. Mendez, N. Molina, E. T. Eng, L. Y. Yen, C. S. Potter, B. Carragher, S. A. Darst

This study uses time-resolved cryo-EM to visualize early intermediates in bacterial RNA polymerase promoter melting, revealing key steps in the formation of the transcription-competent open complex.

bioRxiv · Mar 13, 2024Biophysics

Ensemble cryo-electron microscopy reveals conformational states of the nsp13 helicase in the SARS-CoV-2 helicase replication-transcription complex

J. Chen, Q. Wang, B. Malone, E. Llewellyn, Y. Pechersky, K. Maruthi, E. T. Eng, J. K. Perry, E. A. Campbell, D. E. Shaw, S. A. Darst

This study uses cryo-EM and simulations to identify four conformational states of the nsp13 helicase in the SARS-CoV-2 RTC, revealing an allosteric mechanism for switching between RNA synthesis and backtracking.

bioRxiv · Nov 10, 2021 · PreprintBiophysics

Structural origins of Escherichia coli RNA polymerase open promoter complex stability

R. M. Saecker, J. Chen, C. E. Chiu, B. Malone, J. Sortiris, M. Ebrahim, L. Y. Yen, E. T. Eng, S. A. Darst

This study determines the structural basis of RNA polymerase open complex stability in E. coli by analyzing three promoters with different lifetimes using cryo-EM.

bioRxiv · Sep 8, 2021Biophysics

Structural basis for backtracking by the SARS-CoV-2 replication-transcription complex

B. Malone, J. Chen, Q. Wang, E. Llewellyn, Y. J. Choi, P. D. B. Olinares, X. Cao, C. Hernandez, E. T. Eng, B. T. Chait, D. E. Shaw, R. Landick, S. A. Darst, E. A. Campbell

This paper shows that SARS-CoV-2 RdRp undergoes backtracking, which may aid proofreading and antiviral resistance.

bioRxiv · Mar 13, 2021Biochemistry

Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex

J. Chen, B. Malone, E. Llewellyn, M. Grasso, P. M. M. Shelton, P. D. B. Olinares, K. Maruthi, E. T. Eng, H. Vatandaslar, B. Chait, T. Kapoor, S. A. Darst, E. A. Campbell

The paper presents cryo-EM structures of the SARS-CoV-2 holo-RdRp complex with nsp13 helicase, revealing interactions and a new therapeutic target.

Cell · Sep 1, 2020Peer-reviewedBiophysics

Time-resolved cryoEM using Spotiton

V. P. Dandey, W. C. Budell, H. Wei, D. Bobe, K. Maruthi, M. Kopylov, E. T. Eng, P. A. Kahn, J. E. Hinshaw, N. Kundu, C. M. Nimigean, C. Fan, N. Sukomon, S. Darst, R. Saecker, , B. Carragher

The paper introduces a method for preparing cryoEM grids to study short-lived molecular states by rapidly dispensing sample drops and vitrifying them.

Microscopy and Microanalysis · Jul 30, 2020Peer-reviewedBiophysics