T. Misteli

Allele-level visualization of transcription and chromatin by high-throughput imaging

The paper develops high-throughput DNA/RNA FISH methods to visualize allele-level chromatin features and gene activity across cell populations.

Nuclear position modulates long-range chromatin interactions

The paper investigates how nuclear position affects long-range chromatin interactions by analyzing single-cell data, finding position-dependent interactions that vary by cell type and chromatin type.

Identification of epigenetic modulators as determinants of nuclear size and shape

This study identifies epigenetic modulators that influence nuclear size and shape through a high-throughput siRNA screen and biochemical analysis.

HiFENS: High-throughput FISH detection of endogenous pre-mRNA splicing isoforms

HiFENS is a high-throughput imaging assay that detects endogenous splicing isoforms, used to identify upstream regulators of alternative splicing in human cells.

Structure-function relationships in mitochondrial transcriptional condensates

This study reconstitutes mitochondrial transcriptional condensates in vitro and shows that RNA production alters their phase behavior and leads to non-equilibrium structures.

HiIDDD: A high-throughput imaging pipeline for the quantitative detection of DNA damage in primary human immune cells

HiIDDD provides a high-throughput imaging method to detect DNA damage in primary human immune cells, showing consistent results across cell types and donors.

Large-scale mapping of positional changes of hypoxia-responsive genes upon activation

The paper uses high-throughput imaging to analyze the spatial location of hypoxia-responsive genes and finds that their nuclear position changes upon hypoxia but not directly correlated with gene activity.

Activation of endoplasmic reticulum stress via clustering of inner nuclear membrane proteins

The paper identifies nuclear protein aggregation as a trigger for endoplasmic reticulum stress in Hutchinson-Gilford Progeria Syndrome.

Self-assembly of multi-component mitochondrial nucleoids via phase separation

This study shows that mitochondrial nucleoids assemble via phase separation, with TFAM and mtDNA forming viscoelastic droplets, and links this process to mitochondrial dysfunction in HGPS.

A Deep Learning Pipeline for Nucleus Segmentation

This paper develops a deep learning pipeline to evaluate nuclear segmentation models trained on small, custom annotated datasets, showing they can match or exceed performance of pre-trained models.

Assessment of the Utility of Gene Positioning Biomarkers in the Stratification of Prostate Cancers

This study identifies gene positioning patterns in prostate cancer that correlate with Gleason scores, suggesting spatial genome organization as a prognostic biomarker.

The nucleoporin ELYS regulates nuclear size by controlling NPC number and nuclear import capacity

This study identifies ELYS as a regulator of nuclear size by controlling NPC number and nuclear import capacity in mammalian cells.

HiPLA: High-throughput imaging Proximity Ligation Assay

HiPLA is a high-throughput imaging method for probing protein interactions, revealing differential interactions with the nuclear lamina in HGPS and linking them to expression changes.

Single molecule analysis of lamin dynamics

This study uses single molecule tracking to analyze the dynamics of nuclear envelope proteins and identify how disease mutations affect their behavior.

Effects of Human Sex Chromosome Dosage on Spatial Chromosome Organization

This study investigates how variations in sex chromosome dosage affect chromosome organization in primary cells from SCA patients using HiCTMap imaging.

HiCTMap: Detection and Analysis of Chromosome Territory Structure and Position by High-throughput Imaging

HiCTMap enables high-throughput imaging and analysis of chromosome territories in mammalian cells, providing precise 2D and 3D positioning and quantification.