H. Kimura

Importin α Characterizes a Micronuclear Environment Associated with Genomic Instability in Human Cancer Cells

The paper identifies importin α as a marker of a distinct micronuclear environment in cancer cells, associated with genomic instability and immune evasion.

Reconstruction of artificial nuclei with nuclear import activity in living mouse oocytes

The paper reconstructs artificial nuclei with nuclear import activity in living mouse oocytes using T4 phage DNA, showing they can form functional nuclear structures similar to natural nuclei.

Visualizing histone H4K20me1 in knock-in mice expressing the mCherry-tagged modification-specific intracellular antibody

This paper develops a genetically encoded probe to visualize H4K20me1 in living cells and demonstrates its utility in knock-in mice.

Multiple HP1 binding motifs in SUV420H2 heterochromatic targeting module

This study identifies three HP1 binding motifs in SUV420H2's HTM, showing that the second motif is critical for stabilizing HP1 on heterochromatin.

ISWI chromatin remodeling complexes recruit NSD2 and H3K36me2 in pericentromeric heterochromatin

The paper identifies NSD2 and ISWI chromatin remodeling complexes as mediators of H3K36me2 enrichment in pericentromeric heterochromatin and shows its presence in early mouse embryos.

Precise immunofluorescence canceling for highly multiplexed imaging capturing specific cell state

The paper introduces PECAbs for high-specificity multiplexed imaging of cell states and combines them with seq-smFISH to classify cells and identify signal activation states in human tissue.

Delayed localization of A-type lamins to the rupture sites in Hutchinson-Gilford progeria syndrome

This study investigates the delayed localization of A-type lamins to nuclear envelope rupture sites in HGPS, identifying farnesylation and proteolytic cleavage as key factors affecting this process.

Automated escape system: identifying prey's kinematic and behavioral features critical for predator evasion

This study develops an automated escape system to identify prey kinematic and behavioral features critical for predator evasion, showing that escape speed and reaction distance significantly affect predator-prey interactions.

Histone methyltransferase SUV39H1 regulates the Golgi complex via the nuclear envelope-spanning LINC complex

This study shows that SUV39H1 regulates Golgi dispersion through the LINC complex, linking chromatin organization to cell motility.

Transcription bodies regulate gene expression by sequestering CDK9

This study disrupts transcription bodies in zebrafish embryos and shows they regulate gene expression by sequestering CDK9, affecting both downregulation and upregulation of genes.

Actin filaments accumulate in the nucleus during interphase and remain in the vicinity of condensing chromosomes during prophase to metaphase in zebrafish early embryos

Live-Cell Tracking of γ-H2AX Kinetics Reveals the Distinct Modes of ATM and DNA-PK in Immediate Response to DNA Damage

This study uses live-cell imaging to compare ATM and DNA-PK phosphorylation of H2AX after DNA damage, finding that DNA-PK acts rapidly while ATM shows dynamic chromatin binding.

Nanog organizes transcription bodies

The paper shows Nanog organizes transcription bodies by clustering with Sox19b and RNA polymerase II, requiring its DNA-binding domain and an intrinsically disordered region.

Nucleoplasmic Lamin C Rapidly Accumulates at Sites of Nuclear Envelope Rupture with BAF and cGAS

The paper shows lamin C, BAF, and cGAS accumulate at nuclear envelope rupture sites in mouse cells, with lamin A/C involvement in DNA sensing.

Fractal scaling of C. elegans behavior is shaped by insulin signaling

This study shows insulin signaling in C. elegans regulates fractal scaling of behavior, with glucose and genetic mutants affecting the power-law to exponential transition in residence-time distribution.

Escaping from multiple visual threats: Modulation of escape responses in Pacific staghorn sculpin (Leptocottus armatus).

This study investigates how Pacific staghorn sculpin modulate escape responses to multiple visual threats by varying stimulus timing, finding that modulation occurs only before the response starts.

Visualizing looping of two endogenous genomic loci using synthetic zinc-finger proteins with anti-FLAG and anti-HA frankenbodies in living cells

The paper introduces a method to visualize two endogenous chromatin loci in living cells using synthetic zinc-finger proteins and frankenbodies, revealing their dynamic association and separation upon cohesin disruption.

A Drosophila Toolkit for Imaging of HA-tagged Proteins Unveiled a Block in Autophagy Flux in the Last Instar Larval Fat Body

Chromatin loading of MCM hexamers is associated with di-/tri-methylation of histone H4K20 toward S phase entry

This study investigates MCM hexamer states during the cell cycle in human cells and links their formation to histone H4K20 methylation levels, showing that di/trimethyl-H4K20 correlates with double MCM hexamer formation.

Visualizing transcription sites in living cells using a genetically encoded probe specific for the elongating form of RNA polymerase II

The paper develops a genetically encoded probe to detect elongating RNA polymerase II in living cells and shows its foci move more dynamically than chromatin domains.

RNA polymerase II condensate formation and association with Cajal and histone locus bodies in living human cells

The paper shows that RNA polymerase II condensates form near Cajal and histone locus bodies in human cells, with dynamic interactions during the cell cycle.

Cryptic promoter activation occurs by at least two different mechanisms in the Arabidopsis genome

The paper identifies two mechanisms of cryptic promoter activation in Arabidopsis: chromatin capture of pre-existing promoter-like regions and de novo promoter formation near inserted LUC ORF sequences.

Epstein-Barr virus tegument protein BGLF2 in exosomes released from virus-producer cells assists de novo infection

This study identifies BGLF2, an EBV tegument protein, as being encapsulated in exosomes that enhance viral infection by boosting gene expression and suppressing innate immunity.

Geometric model incorporating prey's turn and predator attack endpoint explains multiple preferred escape trajectories

The paper constructs a geometric model that explains multiple preferred escape trajectories in prey by incorporating prey turning time and predator attack endpoint.

C. elegans episodic swimming is driven by multifractal kinetics

This study identifies multifractal kinetics in C. elegans episodic swimming and links it to PKG regulation of behavioral transitions.

Direct evidence of abortive lytic infection-mediated establishment of Epstein-Barr virus latency during B-cell infection

The paper tracks EBV infection in B-cells using fate mapping and RNA-seq, showing abortive lytic infection precedes latency.

Histone modification dynamics as revealed by a multicolor immunofluorescence-based single-cell analysis

This study introduces a multicolor immunofluorescence method to analyze histone modification dynamics in single cells, revealing changes during the cell cycle and validating histone demethylase targets.

Cohesin and condensin extrude loops in a cell-cycle dependent manner

This study shows that cohesin and condensin extrude distinct types of loops in interphase and metaphase, respectively, revealing cell-cycle dependent loop extrusion dynamics.

Chromatin structure-dependent histone incorporation revealed by a genome-wide deposition assay

The paper develops a method to study histone variant incorporation and finds that chromatin structure and DNA replication influence histone deposition patterns.

Single-molecule imaging reveals control of parental histone recycling by free histones during DNA replication

The paper uses single-molecule imaging in Xenopus laevis egg extracts to show that most parental histones are evicted during DNA replication, with histone recycling and nucleosome sliding occurring less frequently.