Hariprasad Venugopal

This study identifies a high-order ion-coupling mechanism in EAAT transporters by showing that dEAAT2 can acquire Na+, H+, and K+ dependence through mutations, mimicking human hEAAT2's complex ion coupling.

The paper identifies detergent LMNG as stabilizing a weak FZD-G protein interface, revealing that FZDs inherently weakly couple to G proteins and preferentially signal through DVL.

The paper presents cryo-EM structures of the delta opioid receptor with PAMs, identifies a novel allosteric site, and describes a mechanism for enhanced binding and activation.

The paper identifies a conserved TCR motif recognizing mycobacterial lipids presented by CD1c, showing higher frequencies in TB-endemic regions and providing a cryo-EM structure of the interaction.

The paper determines the cryo-EM structure of LRRC15 and identifies the epitope recognized by the antibody samrotamab, revealing a binding mode that preserves signaling surfaces.

PartiNet improves particle picking in cryo-EM with a dynamic neural network that adapts to micrograph quality, enhancing efficiency and particle yield without sacrificing accuracy.

The paper determines the structure of tRNA-bound CRISPR-Cas13 and reveals universal HEPN RNase mechanisms for substrate recognition and catalysis.

This paper presents cryo-EM structures of PORCN with inhibitors, revealing a conserved water-mediated binding mechanism for rational drug design.

This study shows that L-type pyocins inhibit the BAM complex at the cell surface to kill Pseudomonas aeruginosa without entering the cell.

The paper presents cryo-EM structures of the human A3 adenosine receptor in three functional states, revealing a unique activation mechanism and a cryptic binding pocket.

This study designs de novo proteins to inhibit E. coli's heme extraction from hemoglobin, demonstrating their effectiveness at low nanomolar concentrations.

This study characterizes MORC2 phosphorylation's role in DNA compaction and chromatin remodelling, showing it regulates ATP hydrolysis and DNA binding.

The paper identifies PqqU as a high-affinity PQQ importer in Gram-negative bacteria, showing its structural basis and widespread distribution across diverse environments.

The paper presents a cryo-EM structure of the M5 mAChR with a novel allosteric binding site identified through mutagenesis, pharmacology, and simulations.

This study identifies how Mo-CODH and CoxG in Mycobacterium smegmatis oxidize CO and transfer electrons to the respiratory chain via quinone transport.

This paper demonstrates that a standard 120-keV TEM with a direct electron detector can achieve high-resolution cryo-EM reconstructions of various proteins.

The paper presents the structure of Tse15, a T6SS Rhs effector from Acinetobacter baumannii, revealing a novel mechanism for toxin delivery and activation involving auto-cleavage and domain interaction.

This study determines the structure of the human P2X1 receptor in different ligand-bound states and identifies key residues involved in ligand binding and selectivity.

This study elucidates how NanR represses sialic acid metabolism in E. coli by showing its cooperative DNA binding and allosteric regulation by N-acetylneuraminate.

This paper determines the structure of MPEG-1, showing it forms a pre-pore complex and can perforate membranes under acidic conditions.