Application of EGCG modified EDC/NHS cross-linked extracellular matrix to promote macrophage adhesion

Abstract

Though chemically cross-linked by EDC/NHS endows collagen membrane with promising mechanical properties, it is not conducive to modulation of foreign body reaction (FBR) after implantation or guidance of osteogenesis. In our previous research, we have found that macrophages have a strong regulatory effect on tissue and bone regeneration during FBR, and EGCG modified membranes could adjust the recruitment and phenotypes of macrophages. Accordingly, we develop the EGCG-EDC/NHS membranes, prepared with physically immersion, while the surface morphology of the membrane was observed by SEM, the biological activity of collagen was determined by FTIR, the activity and adhesion of cell culture in vitro, angiogenesis and monocyte/macrophage recruitment after subcutaneous implantation, etc. are characterized. It could be concluded that EGCG-EDC/NHS collagen membrane is hopeful to be used in implant dentistry for it not only retains the advantages of the collagen membrane itself, but also improves cell viability, adhesion and vascularization tendency. However, the mechanism that lies in the regenerative advantages of such membrane needs further exploration, but it is certain that the differences in surface morphology can have a significant impact on the reaction between the host and the implant, not to mention macrophage in bone regeneration.