Cryo-EM structure of the human CST·Polα/Primase complex in a recruitment state
S. W. Cai, J. C. Zinder, V. Svetlov, M. Bush, E. Nudler, T. Walz, T. de Lange
Abstract
The CST*Pol/Primase complex is essential for telomere overhang maintenance and additionally functions to counteract resection at double-strand breaks. We report a 4.6-[A] resolution cryo-EM structure of CST*Pol/Primase, captured prior to catalysis in a recruitment state, which provides insights into the architecture and stoichiometry of the fill-in machinery. Our model informs on human disease mutations that cause Coats plus syndrome.
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