fluorescence / live-cell microscopy

This study uses CRISPR/Cas9 to fluorescently tag endogenous IRS2 with an auxin-dependent degron, revealing its dynamic nuclear-cytoplasmic shuttling and role in cancer stem cell function.

This paper develops a single-molecule microscopy assay to measure PETase enzyme kinetics, revealing distinct binding dynamics between wild-type and a hyperactive mutant.

The paper uses fluorescence imaging to show that EGF stimulation causes multiple receptors and regulatory proteins to co-localize at clathrin-coated sites on the plasma membrane of human cells.

This paper develops a flow virometer using confocal fluorescence microscopy to rapidly and specifically detect single nanoparticles and viruses in microfluidic laminar flow.

The paper introduces a multi-parametric particle-based method to analyze organelle landscapes, enabling visualization of heterogeneous organelle structures and transitional states using fluorescence microscopy and UMAP.

This study uses PIE-FCCS to show HER4 directly interacts with all HER family members in live cells, even without ligands, suggesting HER4 is a preferred dimerization partner.

This study develops a fluorescent auxin probe, BODIPY-IAA2, which visualizes auxin distribution in plants without activating auxin signaling.

VISION is an open-source software for automated multi-dimensional image analysis of cellular biophysics, enabling high throughput and correlative analysis of biophysical properties.

This paper introduces CLID techniques for single-frame live-cell super-resolution imaging, achieving 65 nm resolution without complex parameters.

This paper introduces SN2N, a self-supervised denoising method for live-cell super-resolution microscopy that achieves competitive performance without requiring large clean datasets.

navigate provides an open-source platform for smart light-sheet microscopy, enabling automated and user-friendly LSFM workflows without requiring specialized expertise.

The paper develops a machine learning method to recognize individual F-actin filaments in HS-AFM images, revealing their orientation patterns in lamellipodia and cell cortex.

This study uses MINFLUX to track dynein movement in live neurons, revealing 8 nm steps and rapid direction reversals, suggesting a single ATP-dependent stepping mechanism.

This paper develops and applies species-specific rRNA-FISH to study interspecies material exchange and population dynamics in clostridial co-cultures, revealing hybrid cell formation and distinct translation machinery localization.

The paper identifies a thermostable fluorescent protein, TGP, for live imaging in Sulfolobus acidocaldarius, enabling protein localization at high temperatures previously unattainable with mesophilic proteins.

This study uses a biomimetic system to show that E-cadherin mobility on lipid bilayers influences actin polymerization, leading to distinct actin architectures during adherens junction formation.

The paper quantifies redox signals and photosynthetic activity in potato plants using time and wavelength-resolved fluorescence spectra, revealing a link between electron transport rate and oxidative signals.

This study examines how stress conditions alter the structure and function of membrane-based organelles in budding yeast using fluorescent markers.

CellSeg3D introduces self-supervised 3D cell segmentation models for fluorescence microscopy, achieving performance comparable to supervised methods without requiring ground truth labels.

This paper introduces a spectral method with neural networks to detect chromatin loops in multiplexed super-resolution FISH data, validated on published datasets.

This paper introduces SINCS, a compression method for multidimensional biomedical images that improves efficiency and quality by leveraging semantic redundancy and saliency maps.

This study shows that Pseudomonas aeruginosa uses spatial sensing, not temporal sensing, to detect chemical gradients during surface-based chemotaxis.

This paper introduces a novel image clustering approach for CAR-T/tumor cocultures using U-net and clustering algorithms, demonstrating superior performance in classification and data interpretation.

This study applies FastSAM for automated algal cell segmentation and clustering in water samples, showing high accuracy for single cells and varying clustering performance.

The paper shows that RNase L-induced bodies sequester subgenomic flavivirus RNAs, reducing their interaction with P-bodies and enhancing viral RNA decay.

The paper introduces da_Tracker, an automated workflow for high-throughput single-cell and single-phagosome tracking in infected cells, enabling quantitative analysis of pathogen-host interactions.

This paper develops a time-resolved fluorescence resonance energy transfer (TR-FRET) assay for screening small molecule inhibitors targeting the SYK and FCER1G interaction, identifying hematoxylin as a hit.

This study measures the kinetics of SARS-CoV-2 virus-like particle internalization using live-cell microscopy, revealing key steps and their timing, and showing no effect of Omicron mutations or S protein cleavage site on internalization rates.

This paper develops genetically encoded fluorescent sensors to measure polyamine levels, uptake, and distribution in cells, revealing compounds that modulate polyamine levels.

This study compares the nuclear dynamics of Bicoid and Capicua in Drosophila embryos, finding they form similar oligomeric clusters despite opposing transcriptional roles.