Microbial Natural Products and Biosynthesis

This paper reports a new indolocarbazole biosynthetic pathway in Streptomyces venezuelae and identifies a regioselective C-5 hydroxylating enzyme.

This paper identifies a novel aminosugar metabolic pathway in Streptomyces that regulates development and antibiotic production through GlcNAc sensing.

The paper identifies a new family of RiPPs in lactic acid bacteria biosynthetic gene clusters, revealing a novel adenylyltransferase mechanism for macrocyclic imide formation.

The paper determines the structure of the thioesterase domain of SulM and its complex with the upstream carrier protein domain, revealing a constrained active site for β-lactam formation.

This study identifies and characterizes transcriptional regulators involved in the biosynthesis of brasilicardin in Nocardia terpenica, revealing their regulatory roles and interactions.

This paper improves the expression of a type III polyketide synthase in cell-free systems by optimizing codon usage and promoters, showing better results than traditional codon optimization.

The paper provides over 1,000 high-quality Actinomycetia genomes, revealing genomic structures and biosynthetic gene cluster organization with ecological and biotechnological implications.

This study identifies a lantibiotic dehydratase that catalyzes peptide bond formation in the opposite direction of NRPS and PKS biosynthesis, revealing its role as a separate amide bond-forming machinery.

This study uses single-molecule microscopy to show that PvdL is localized at the inner membrane and co-localizes with other NRPSs, suggesting it orchestrates their assembly in pyoverdine biosynthesis.

The paper identifies and characterizes enzymes involved in N-hydroxypyrrole biosynthesis, revealing new insights into the formation of unique functional groups in natural products.

This study uses genomic and bioinformatic analysis to classify five Streptomyces species from the Himalayas and identify their biosynthetic potential for antibiotic production.

This study characterizes the porcine skin microbiome, identifies antifungal bacterial isolates, and discovers novel fungal and bacterial species with potential for antifungal applications.

This study analyzes PTM biosynthetic clusters to uncover their phylogenetic diversity and functional complexity, revealing gaps in bioinformatics predictions and highlighting challenges in genomic discovery.

The paper identifies a consistent specialized metabolome in Trichodesmium thiebautii blooms and isolates a new macrocyclic compound, suggesting potential ecological roles for these metabolites.

This study evaluates the antioxidant and anti-inflammatory potential of an endophytic Bacillus haynesii from Momordica charantia and identifies its bioactive metabolites.

The paper reports the discovery of maramycin, a novel isoquinolinequinone alkaloid produced through heterologous expression of a bifunctional enzyme in Streptomyces albidoflavus, showing moderate cytotoxicity against prostate cancer cells.

This paper investigates how DarE enzyme modifies DarA to form ether and C-C crosslinks, identifying key residues and mechanisms involved in radical-mediated biosynthesis.

This study reveals the complexity of pyrroloiminoquinone biosynthesis by identifying four additional enzymes that correct a previously proposed pathway and show that amino groups come from glycine, asparagine, and leucine.

This study creates novel hybrid aminoglycoside antibiotics by swapping glycosyltransferases between gentamicin and kanamycin producers, resulting in compounds with potent activity and reduced ototoxicity.

The paper identifies residue E299 as critical for the divergent catalytic functions of DnrK and RdmB in anthracycline biosynthesis, revealing distinct mechanisms for 10-hydroxylation and 4-O-methylation.

The paper updates a genome mining tool to identify borosin precursor peptides and characterizes new biosynthetic features.

The paper identifies and characterizes the chm biosynthetic gene cluster responsible for chalkophomycin production in Streptomyces sp. CB00271 and proposes a biosynthesis model based on sequence analysis and structural modeling.

This study identifies umbrella toxin particles produced by Streptomyces that inhibit the growth of competing bacterial species, distinct from small molecule antibiotics.

This study shows that nutrient deprivation in Streptomyces triggers a foraging growth phenotype that enhances antimicrobial activity and secondary metabolite production.

BGCFlow is a systematic workflow for analyzing biosynthetic gene clusters across large genomic datasets, demonstrating improved dereplication and comparative analysis of RiPPs in Saccharopolyspora genomes.

The paper identifies the biosynthetic pathway for 3-NPA in Aspergillus oryzae using genome mining, genetic inactivation, and biochemical analysis.

SYNTERUPTOR identifies genomic islands containing specialised metabolite gene clusters in microbial genomes, detecting clusters missed by existing tools.

This study develops new genetic tools for Photorhabdus and Xenorhabdus, including SEVA-based expression vectors and CRISPR/Cpf1 genome editing systems, enabling efficient natural product profiling.

This study compares molecular functions and biosynthetic gene clusters in cyanobacterial genomes to identify specializations associated with symbiotic lifestyles.