RNA Interference and Gene Delivery

This paper optimizes RNA interference protocols in Macrostomum lignano by demonstrating that single soaking in dsRNA solution is sufficient for effective gene knockdown, reducing time and cost.

This study evaluates a 16-residue peptide from Medicago truncatula defensin for its ability to penetrate mammalian cells and deliver cargo, showing efficient uptake and minimal cytotoxicity.

This study conjugates synthetic siderophores to antisense oligomers to deliver them into bacteria, showing potent antibacterial effects without mammalian toxicity.

The paper shows that HRDE-2 mediates the localization of HRDE-1 to germ granules, which is essential for its specific binding to small RNAs and proper gene silencing in C. elegans.

This study compares the efficiency of cell-penetrating peptides and cationic liposomes for delivering anti-BCR-ABL siRNA to inhibit CML cell growth in vitro.

The paper demonstrates that endosomolytic peptides L17E and L17ER4 effectively deliver PNAs into human cells, enabling corrective splicing.

This paper develops DC-targeted polymeric nanoparticles for mRNA vaccine delivery, showing enhanced immune responses in mice compared to traditional LNPs.

This study shows that Dicer{Delta}HEL1/wt mice exhibit increased endogenous siRNA levels and functional RNAi in vivo, providing a new model for studying mammalian canonical RNAi.

This study investigates the cellular uptake and fate of cationic polymer-coated nanodiamonds delivering siRNA, finding clathrin-mediated endocytosis as the main entry mechanism and effective gene silencing with low toxicity.

This study evaluates CaCO3-based nanoparticles delivering miR-200c to suppress OSCC growth and metastasis in vitro and in vivo.

This paper develops a new mRNA-BLNP3 nanoparticle that selectively delivers mRNA to antigen-presenting cells, showing improved immune responses and reduced liver accumulation compared to previous versions.

This paper introduces a dual-targeting di-valent siRNA scaffold that can silence two genes simultaneously in the CNS of mice, demonstrating potent and programmable multi-gene modulation.

This study develops an optimized siRNA transfection protocol for human and mouse adipocyte cultures throughout differentiation, improving gene knockdown efficiency.

This study evaluates tetravalent siRNA configurations for photoreceptor gene silencing, finding that tetravalent siRNA provides robust, long-lasting efficacy with a good safety profile in rodent and swine models.

The paper shows AGO2 localizes to the nucleus in quiescent cells to silence mobile transposons via RNA interference.

The paper determines the minimum guide lengths required for miR-20a and let-7a to maintain their RISC conformation, finding 19 and 20 nt respectively, and identifies 17-nt guides as functional tinyRNAs (tyRNAs) without PAZ domain anchoring.

This paper uses machine learning to predict the effectiveness of ionizable lipids for mRNA delivery, identifying the number of outside carbons as a key factor.

The paper presents cryo-EM structures of human SIDT1 and SIDT2, revealing their dimeric architecture and demonstrating low-pH-dependent RNA binding and oligomerization.

DeepSipred predicts siRNA inhibition using sequence, thermodynamic, and expert knowledge features, achieving state-of-the-art performance on public datasets.

This study systematically compares siRNA delivery methods to T cells, finding lipid-conjugate-mediated delivery is most effective for both resting and activated T cells ex vivo.

This paper introduces ionizable lipids with dual ethanolamine head groups for phospholipid-free mRNA delivery, showing improved stability, efficiency, and reduced toxicity.

This paper demonstrates the effective long-distance transport and gene silencing in plants using cationized bovine serum albumin (cBSA) bound to double-stranded RNA nanocomplexes.

The paper optimizes RNAi efficiency in C. elegans PVD neurons by expressing sid-1 under the mec-3 promoter, enhancing gene knockdown effects for dendrite development and regeneration studies.

This study uses machine learning to predict the silencing activity of chemically modified siRNAs based on sequence and modification patterns, showing improved performance over existing methods.

This paper demonstrates that a cardiomyocyte-targeting peptide can deliver amiodarone at a lower dose, reducing cardiac conduction velocity and action potential duration with fewer side effects.

This paper introduces ARTi, a method using synthetic shRNAs to achieve precise and controllable RNAi for gene suppression.

This study shows that recombinant Ranpirnase enhances mRNA transgene expression in mice by 3-6 fold and prolongs its duration by over 85% without cytotoxicity.

This paper develops 2'-OMe/LNA oligonucleotides to selectively inhibit specific miRNA precursors, demonstrating in vitro and in vivo target selectivity for miRNA family members.

The paper identifies TRIM71's mechanism of mRNA silencing through recruitment of TNRC6/GW182 and shows competition among silencing pathways.

This paper designs potent siRNAs against SARS-CoV-2 using biochemistry-informed computational methods, achieving high antiviral activity and variant coverage.