Extracellular vesicles in disease

This paper introduces a method for isolating surface-engineered extracellular vesicles using TFF-SEC, showing improved purity and efficiency compared to conventional methods.

This study shows that pneumolysin-loaded host extracellular vesicles contribute to pneumococcal pathogenesis by inducing cytotoxicity and inflammation.

This study shows how engineering protein-lipid interactions can improve the loading and delivery of protein cargo into extracellular vesicles for therapeutic applications.

This study shows that circulating tumor extracellular vesicles can be used to monitor genomic and transcriptomic changes in metastatic prostate cancer through liquid biopsy.

The paper develops DETECTOR-seq to analyze fragmented cell-free RNAs in plasma and extracellular vesicles, revealing distinct RNA distributions and potential biomarkers for cancer detection.

The paper develops a HaloTag-based system for quantitatively characterizing engineered proteins on extracellular vesicles and compares it to antibody labeling.

This study shows that mesenchymal stem cell-derived extracellular vesicles reduce hippocampal NLRP3 inflammasome activation and inflammation in adolescent mice after binge ethanol treatment.

This paper introduces a fast and sensitive size-exclusion chromatography method for plasma extracellular vesicle proteomic analysis, demonstrating its utility in identifying EV proteins related to physical activity.

The paper identifies a 5-gene exosomal biomarker panel with high accuracy for predicting liver metastasis in lung cancer patients.

The paper quantifies secreted and extracellular vesicle (EV) urinary proteomes in men with and without prostate cancer, showing their stability and utility in distinguishing malignant from benign lesions.

The paper assesses BEV-enrichment protocols for blood biomarkers in neurodegenerative diseases and identifies CNS-specific proteins for enrichment and biomarker use.

This paper uses magnetic particle imaging to show that iron-labeled extracellular vesicles accumulate in brains of mice with brain metastases but not in mice without metastases.

The paper develops a method to enrich cells with high extracellular vesicle secretion and shows improved therapeutic outcomes in a mouse model.

This study develops red blood cell-derived extracellular vesicles loaded with miR-204 mimic to treat neuroblastoma, showing inhibitory effects on cell viability and spheroid growth.

This study shows hypoxic tumor cells release extracellular vesicles containing ALS2, which activate Rab5 in endothelial cells to promote angiogenesis via β-catenin stabilization.

This study shows that extracellular vesicles from human oral mucosa stem cells promote autophagy in human microglia via lipid raft-dependent mechanisms involving TLR4, HSP70, integrins, and P2X4R.

This paper introduces a novel immunoassay for detecting EGFR and its activity state in plasma CD63-EVs from glioblastoma patients, achieving high sensitivity and specificity for early detection.

This study identifies ciliary intrinsic mechanisms that regulate dynamic extracellular vesicle release from sensory neurons in C. elegans, showing that KLP-6 and TTM-5 are required for sustained release.

This study compares protein cargo differences in mesenchymal stem cell-derived extracellular vesicles isolated by three methods, finding method-specific impacts on proteome composition and biological function.

This paper shows that stress-induced Rab11a-exosomes carrying AREG promote cetuximab resistance in colorectal cancer by activating EGFR signaling in KRAS-wild type cells.

This paper introduces Membrane-Sensing Peptides (MSP) as universal affinity ligands for analyzing Extracellular Vesicles and analogues, demonstrating their ability to detect EV-associated epitopes in clinical samples without prior isolation.

The paper shows that ESCRT disruption in Drosophila motor neurons affects EV cargo release but not their signaling activities, suggesting EVs may primarily serve proteostatic functions.

The paper demonstrates that TRAIL-loaded exosomes from induced neural stem cells effectively target and kill brain cancer cells in vitro and in vivo.

This study uses chiral quantum dots to investigate origin-dependent exosome uptake and cargo release mechanisms in different recipient cells.

This study shows that syntenin and CD63 promote exosome biogenesis by blocking CD63 endocytosis, allowing it to accumulate at the plasma membrane where exosomes originate.

The paper uses a single-cell transcriptomic atlas to predict cell type-specific extracellular vesicle biology, identifying gene expression patterns and networks associated with EV secretion.

The paper introduces a novel pH-based method for loading therapeutic toxins into extracellular vesicles, preserving their properties and enhancing cargo activity.

The paper identifies APE1-dependent miRNAs secreted via exosomes, showing their prognostic significance in cancer and their regulation by APE1 and hnRNPA2B1.

The paper uses cryo-TEM and a segmentation model to analyze EV architecture, finding consistent structural heterogeneity across different sources and purification methods.

The paper identifies miRNAs selectively loaded into extracellular vesicles from healthy human astrocytes and links them to neurodevelopmental disorders.