Telomeres, Telomerase, and Senescence

This study identifies a RPA-Trt1TERT-Tpz1TPP1 complex in fission yeast that promotes telomere extension and suppresses recombination through specific protein interactions.

The paper identifies heritable telomere variant sequences that determine allele-specific telomere length and shows their role in telomere maintenance and inheritance.

This study introduces PreCSenM, a machine learning model that quantifies cellular senescence and identifies HDAC inhibitors as senescence inducers in lung adenocarcinoma.

This paper introduces FAβ-gal, a fluorescence-based method to quantify SA-β-gal activity using X-gal, improving accuracy and sensitivity over traditional colorimetric assays.

This study shows that cfDNA can map telomere chromatin structure, revealing age-related and disease-specific changes in telomeric footprints and gene signatures.

This study shows Cdc13 initially binds telomeres as a monomer, then forms a stable dimer, revealing a sequential mechanism for telomere protection and length regulation.

This paper shows that tumour telomere length is influenced by patient-specific telomere length, replicating this across two cancer cohorts.

This study investigates the role of human PIF1 in ALT telomere maintenance, showing its involvement in DNA damage, G4 formation, and replication stress response, with implications for cancer therapy.

This study identifies mitochondrial dysfunction as a key feature of brain senescence in telomerase-deficient mice, linking telomere attrition to impaired mitochondrial function and region-specific neuronal vulnerability.

This paper develops a standardized in vitro model for senolytic drug screening using human intervertebral disc cells and validates a metabolic activity assay as a scalable method to assess senolytic activity.

The paper identifies a mechanism of gene amplification in yeast involving microhomology-mediated break-induced replication (mmBIR) at telomeres, leading to tandem duplication of chromosomal ends.

This study identifies two HES1-expressing senescent CTC subpopulations with distinct metabolic and clinical outcomes, showing HES1-SOD1 antagonism influences metastatic relapse.

This study shows that targeting CCND1-CDK6 reduces DNA damage, inflammation, and age-related decline in mice by suppressing SASP and ISG expression.

This paper introduces a method using Oxford Nanopore long-read sequencing to measure chromosome-specific telomere lengths in human cells.

This study identifies the BLM DHBN domain and the roles of WRN, MUS81, and GEN1 in mitotic arrest-dependent telomere deprotection, showing TRF2's protective role.

The paper analyzes the transcriptomic effects of PCC1 treatment on senescent human stromal cells, identifying gene expression changes in ribosomal, lysosomal, and membrane-lipid pathways.

This study shows that cellular senescence disrupts liver zonation and hepatocyte function with age by altering gene expression and cell signaling.

This study investigates rapid evolution of TERT in Mimulus lewisii and shows its functional divergence in binding TR paralogs, leading to telomere sequence changes and dominance in hybrids.

This study shows that early suppression of the pro-inflammatory senescent secretome (SASP) with ruxolitinib reduces chronic bone damage after radiotherapy.

This paper identifies AtLa1 as an essential RNA-binding factor for Arabidopsis telomerase function, revealing its role in telomerase complex assembly and highlighting evolutionary conservation with yeast and ciliate proteins.

The paper identifies cell-type-specific SASP ligand-receptor pairs that mediate paracrine senescence spreading in human brain cells and shows that targeting these factors can prevent senescence spread.

The paper shows that disease mutations in dyskerin's N-terminal extension and α2-helix do not prevent dimerization but impair hTR binding, contributing to X-DC pathogenesis.

This study identifies LINE-1 retrotransposons as drivers of neuronal senescence in Alzheimer's disease through cytoplasmic DNA sensing and the cGAS-STING pathway.

The paper shows that human-length telomeres in mice restrict hematopoietic stem cell regenerative potential under stress conditions.

The paper introduces a CRISPR/Cas9 system in S. pombe to study telomere replication, revealing RNase H2-dependent RNA primer removal in lagging-strand telomeres.

This paper identifies senescence-induced CTCF clusters (SICCs) and shows they sustain the senescence splicing program by repurposing nuclear speckle components.

This paper introduces a live-cell imaging protocol using LysoTracker Deep Red to quantify lysosomal remodeling as a marker of cellular senescence in human fibroblasts.

This study characterizes TERRA and ARIA telomeric RNAs in CHO cells, showing ARIA regulates telomeric DNA integrity by preventing ssDNA formation at ITSs.

This study shows that oncogene inactivation-induced senescence both limits tumor growth and promotes relapse by creating a pro-recurrence microenvironment.

The paper identifies Esc1-mediated telomere anchoring as a mechanism regulating telomere clustering in yeast during metabolic shifts to quiescence.