Ubiquitin and proteasome pathways

The paper investigates how ZFAND5 activates the 26S proteasome by inducing conformational changes that enhance substrate degradation.

This study identifies HAPSTR1 as a HUWE1 substrate that facilitates its nuclear localization, thereby regulating stress signaling pathways and cell proliferation.

The paper identifies RNA polymerase I as a target of Nse1's RING domain through ubiquitin proteomics, revealing a role in rDNA segregation.

This study identifies a bi-functional N-terminal degradation control sequence in REV-ERBα that regulates its ubiquitination and degradation through interactions with E3 ligases.

The paper identifies 15 genomic loci influencing proteasome activity in yeast using single-cell measurements and CRISPR-based engineering, revealing substrate-specific genetic effects.

This study shows that immunoproteasome deficiency accelerates brain aging and causes epilepsy in mice, with reduced immunoproteasome expression also observed in human epilepsy patients.

The paper identifies lysine deserts in UPS components across species and shows they can be regulated by non-lysine ubiquitination and ubiquitin-independent proteasomal degradation.

This study shows neuronal membrane proteasomes regulate neural activity and are essential for learning-induced behavioral plasticity in Xenopus laevis tadpoles.

This study shows that sumoylation is largely dispensable for normal yeast growth but essential for heat tolerance, as reduced Ubc9 activity leads to temperature sensitivity and transcriptional dysregulation.

The paper identifies genetic variants affecting UPS activity in yeast by mapping loci influencing N-degron recognition and ubiquitination.

The paper identifies a small molecule DUB probe, IMP-2373, that quantitatively engages multiple DUBs in live cells and demonstrates its use in studying DUB activity changes during MYC deregulation.

This study investigates how mutations at alanine-126 in helix-3 of Rad6 affect its ubiquitination activity and structural stability in yeast and human homologs.

The paper characterizes the differential distribution and targets of Sumo paralogs in the mouse brain using knock-in mouse models and proteomic analysis.

The paper predicts SUMOylation targets in Drosophila melanogaster using sequence homology from human and mouse SUMOylation data, identifying motifs, protein families, and functional preferences.

This study investigates the germline-specific functions of 19S RP proteasome subunits in C. elegans, revealing roles in meiotic processes and proteasome assembly.

The paper shows that UBX proteins lower the ubiquitin threshold for p97-UFD1-NPL4 to unfold substrates, similar to yeast.

This study shows TRIM21 can degrade lysine-less substrates without autoubiquitination, revealing a non-canonical ubiquitination mechanism for Trim-Away.

The paper investigates how the CHN-1/UFD-2 complex enhances ubiquitin chain formation through dimerization and chaperone-mediated regulation of substrate ubiquitylation.

This study identifies UBE2 family members that interact with MuRF1 to mediate ubiquitylation, revealing a two-step mechanism for mono- and poly-ubiquitin chain formation.

The paper shows that ETC-1 promotes proteasome processivity to degrade oocyte regulatory proteins during the oocyte-to-embryo transition in C. elegans.

The paper evaluates twenty-four anti-SUMO monoclonal antibodies for their sensitivity, specificity, and ability to detect different SUMO conjugation states across multiple assays.

This study identifies RTL8 as a novel UBQLN2 interactor that promotes its nuclear translocation and subnuclear localization in protein quality control.

This paper determines the crystal structure of KLHL3 in complex with a WNK3 peptide, revealing structural features and phosphorylation-dependent regulation of WNK3 binding.

The paper introduces a computational tool to analyze ubiquitin E3 ligase activity using quantitative proteomics data and validates it with experimental results.

This study identifies an FBXO7 mutation that disrupts its interaction with PI31, affecting proteasome and mitochondrial function in patient cells.

ReporterSeq identifies Trr1 and ribosomal proteins as novel regulators of the UPS master regulator Rpn4 in yeast under stress conditions.

This study shows that ATR promotes Api5 degradation via the ubiquitin-proteasome pathway through FBXW2, thereby regulating apoptosis in response to DNA damage.

The paper presents the cryo-EM structure of HUWE1, revealing its modular architecture and its role in degrading cell-fate decision factors.

The paper identifies a conserved Naa40p proteoform, Naa40S, which exhibits distinct substrate specificity and subcellular localization compared to Naa40L, expanding the known substrate repertoire of Naa40p.

This study investigates the impact of Rpt6 phosphorylation on synaptic plasticity and learning using mouse models, finding no significant effects on synaptic function or behavior.