Nuclear Structure and Function

This paper introduces pan-Expansion Microscopy to visualize nuclear pore complex plasticity, revealing LINC complex-dependent diameter biases and nucleoporin repositioning in an ALS model.

This study reconstitutes a nucleus in giant unilamellar vesicles using Xenopus egg extract, demonstrating functional nuclear import and pore complexes.

The paper shows that acute chromatin decompaction increases nuclear stiffness in some cells but not others, depending on their ability to recompact chromatin.

This study models lamin aggregation and dissociation in progeria using polymer chains to show how molecular interactions and concentration affect nuclear lamina structure and stability.

This study shows that nuclear pore complexes dilate during cell differentiation and that Nup133 deficiency prevents this dilation, leading to nuclear envelope instability.

This study shows that RG repeats in C. elegans can accumulate in nucleoli but not form specific sub-nucleolar compartments.

The paper identifies a novel phosphorylated NES in Pho4 recognized by Msn5, revealing a phosphate-specific nuclear export mechanism in yeast.

This study identifies Ankle2 as a regulatory subunit of PP2A that promotes BAF dephosphorylation and nuclear reassembly by interacting with Vap33 during telophase.

A whole genome multiparametric CRISPR screen in progeria identifies new pathways that can reverse cellular aging phenotypes by targeting protein translation and related processes.

The paper uses expansion microscopy and proximity labeling to reveal an asymmetric nuclear pore structure in trypanosomes, identifying unique and conserved components involved in mRNA export.

The paper investigates how nuclear blebs alter chromatin packing domains, finding fragmentation of nanoscopic heterochromatin domains within blebs.

This study identifies the sequence specificity of Mcm3's NLS using mutagenesis and AlphaFold 3, showing that precise basic residue positioning is critical for nuclear import and chromatin loading.

This study investigates how HIV-1 capsids pass through nuclear pores, finding that capsids retain their structure and cause NPC scaffold rings to crack.

The paper examines emerin expression in breast cancer subtypes and finds it is reduced in aggressive subtypes, correlating with advanced stage, higher proliferation, and receptor-negative status.

The paper identifies two nuclear localization signals in the Anelloviridae capsid protein ORF1, showing their distinct roles in subcellular localization and evolutionary adaptation.

The paper quantifies the lamin A/C distribution during cell migration and aspiration through constrictions, showing the lamin network passively responds to nuclear squeezing.

The paper uses NEED-seq to show lamin B1 and B2 associate with distinct heterochromatin states and are linked to neuronal development.

This study shows that Dictyostelium discoideum uses nuclear pores as conduits for fluid flow during osmotic stress, distinct from passive diffusion and active transport.

This study shows DNA damage reduces heterochromatin via ATM, leading to nuclear softening, blebbing, and rupture in MEF cells.

This study shows progerin induces DNA damage without affecting replication or cell proliferation in human cells.

This study identifies RNA-dependent PIP2-associated proteins in human cells and shows their role in nuclear compartmentalization through phase separation.

This study investigates the delayed localization of A-type lamins to nuclear envelope rupture sites in HGPS, identifying farnesylation and proteolytic cleavage as key factors affecting this process.

The paper identifies an interkinetic envelope surrounding chromosomes in C. elegans oocytes during interkinesis, showing it is a distinct structure from the nuclear envelope.

The paper investigates how varying the width of DNA origami-based nuclear pore mimics affects permeability, showing that narrower pores restrict viral capsid passage while wider pores allow it with NTR assistance.

This study uses U-ExM and RITE to show Nup221 turnover in P. berghei NPCs during blood-stage replication.

The paper compares lamin B and DNA levels in nuclear blebs across cell types and perturbations, finding DNA concentration is more consistently reduced than lamin B levels.

The paper identifies NMCP-class nuclear lamina proteins in streptophytic algae using structural features, finding structural variance and absence in Mesostigmatophyceae, and linking presence to multicellularity.

This study shows that increased Lamin A expression reduces nuclear deformability and impairs apical nuclear migration in zebrafish retinal neuroepithelium.

This study reveals how Kap114 and Nap1 co-chaperone H2A-H2B during nuclear import and nucleosome assembly through structural and biochemical analyses.

This study shows WRN helicase maintains constitutive heterochromatin in human fibroblasts by interacting with HDAC2 and HP1, and its loss disrupts heterochromatin structure and LBR expression, contributing to aging and senescence.