A. Jegou

Regeneration of actin filament branches from the same Arp2/3 complex

The paper shows that Arp2/3 complex can re-nucleate actin branches without NPF activation after debranching, suggesting a self-repair mechanism for actin networks.

Cortactin stabilizes actin branches by bridging activated Arp2/3 to its nucleated actin filament

This study reveals how cortactin stabilizes actin branches by interacting with activated Arp3 at the branch site, displacing other nucleation promoting factors.

Fascin-induced bundling protects actin filaments from disassembly by cofilin

This study shows that fascin crosslinking slows cofilin-induced actin filament disassembly by reducing cofilin cluster nucleation on bundles, while promoting inter-filament cooperativity.

Histidine 73 methylation coordinates ????-actin plasticity in response to key environmental factors

This study uses molecular dynamics simulations to show that H73 methylation in β-actin affects nucleotide binding, allosteric pathways, and phosphate release, regulating actin plasticity in response to environmental factors.

Direct observation of the conformational states of formin mDia1 at actin filament barbed ends and along the filament.

This study uses electron microscopy to directly observe two conformations of mDia1 FH2 domains interacting with actin filament barbed ends, supporting the stair stepping model.

Nucleation and stability of branched versus linear Arp2/3-generated actin filaments

This study compares the nucleation and stability of branched versus linear actin filaments generated by Arp2/3 complex activation by VCA domains or SPIN90, revealing similar regulatory responses but distinct mechanical and temporal stability.

Dynamics of Tpm1.8 domains on actin filaments with single moleculeresolution

This study uses fluorescence microscopy and modeling to quantify Tpm1.8 binding kinetics on actin filaments, revealing independent decoration of actin strands and asymmetric domain dynamics.

IN VITRO RECONSTITUTION OF DYNEIN FORCE EXERTION IN BULK CYTOPLASM

This study demonstrates that cargo motion in bulk cytoplasm exerts forces on microtubules, revealing how cargo size and MT length influence force efficiency and enabling tug-of-war dynamics.

Actin filament oxidation by MICAL1 suppresses protections from cofilin-induced disassembly

This study shows that MICAL1-induced actin filament oxidation enhances cofilin binding and severing, bypassing the need for cofilin activation and overcoming tropomyosin protection.

Twinfilin uncaps filament barbed ends to promote turnover of lamellipodial actin networks

This study identifies twinfilin as a regulator of capping protein dynamics in actin networks, showing it promotes CP dissociation and filament turnover in cells.

Geometrical constraints greatly hinder formin mDia1 activity

This study shows that geometrical constraints significantly reduce mDia1 formin elongation rates and processivity when filaments are bundled or cross-linked.

Quantitative variations of ADF/cofilin’s multiple actions on actin filaments with pH

This paper quantifies how ADF/cofilin's actions on actin filaments change with pH, showing that pH affects filament severing rates but not severing per domain boundary, and that barbed-end depolymerization remains unstoppable at physiological pH.

Torsional stress generated by ADF/cofilin on cross-linked actin filaments boosts their severing

This study shows that ADF/cofilin-induced torsional stress enhances actin filament severing in cross-linked networks by increasing the severing rate constant 100-fold.

Modulation of formin processivity by profilin and mechanical tension

This study investigates how profilin and mechanical tension modulate formin mDia1 processivity by altering dissociation rates from actin filaments.