J. Walsh

Tumor cell-based liquid biopsy using high-throughput microfluidic enrichment of entire leukapheresis product

This study uses high-throughput microfluidic technology to enrich CTCs from large blood volumes, enabling detailed molecular and morphological analysis of tumor cells in metastatic cancer patients.

An exploration of mechanisms underlying Desemzia incerta colonization resistance to methicillin-resistant Staphylococcus aureus on the skin

This study identifies Desemzia incerta as a skin commensal that reduces MRSA colonization through secreted antimicrobial proteins and decreased biofilm formation in a murine model.

Harnessing diversity and antagonism within the pig skin microbiota to identify novel mediators of colonization resistance to methicillin-resistant Staphylococcus aureus

The paper identifies novel microbiota-mediated mechanisms of colonization resistance to MRSA in pig skin using a swine model and isolates.

Spatial variation in population genomic responses to over a century of anthropogenic change within a tidal marsh songbird

The paper documents population genomic responses to habitat loss in Savannah sparrows over 128 years, finding that genetic diversity was not correlated with habitat loss and that immigration mitigated diversity loss but reduced divergence at adaptive loci.

Piezo buffers mechanical stress via modulation of intracellular Ca2+ handling in the Drosophila heart

This study shows that Piezo channels in Drosophila hearts modulate intracellular Ca2+ handling to buffer mechanical stress and prevent arrhythmias.

Single-molecule analysis of the entire perfringolysin O poreformation pathway

This study uses single-molecule fluorescence imaging to analyze the kinetics of PFO pore formation, revealing that membrane insertion is kinetically controlled.

Adaptive divergence in shoot gravitropism creates hybrid sterility in an Australian wildflower

This study shows that adaptive divergence in shoot gravitropism contributes to hybrid sterility in Senecio lautus, linking local adaptation to speciation through reproductive isolation.

Development of a method for revertable CRISPR/Cas9-based mutagenesis in cell culture

The paper develops a CRISPR/Cas9 method to create revertable mutations in cell culture by inserting a LoxP-flanked stop sequence via HDR, allowing restoration of gene function.

Dynamics of Tpm1.8 domains on actin filaments with single moleculeresolution

This study uses fluorescence microscopy and modeling to quantify Tpm1.8 binding kinetics on actin filaments, revealing independent decoration of actin strands and asymmetric domain dynamics.

Fluorescence biosensor for real-time interaction dynamics of host proteins with HIV-1 capsid tubes

This paper develops a fluorescence biosensor to quantify host protein interactions with HIV-1 capsid tubes in real-time using total internal reflection fluorescence microscopy.

Divergent selection and drift shape the genomes of two avian sister species spanning a saline-freshwater ecotone

This study compares the genomes of two avian sister species to assess the roles of divergent selection and genetic drift in shaping their divergence across a saline-freshwater ecotone.