A. Schepartz

Requirements for efficient endosomal escape by designed mini-proteins

This paper identifies the requirements for efficient endosomal escape by ZF5.3, a mini-protein, showing pH-induced unfolding and lipid-BMP interaction are critical for its function.

β-amino acids reduce ternary complex stability and alter the translation elongation mechanism

This study investigates how β-amino acid isomers affect ternary complex stability and translation elongation by analyzing their impact on ribosome function and EF-Tu interactions.

Design rules for efficient endosomal escape

This paper identifies design rules for efficient endosomal escape by showing that protein size and thermal stability influence delivery efficiency when conjugated to ZF5.3.

A Bright, Photostable Dye that Enables Multicolor, Time Lapse, and Super-Resolution Imaging of Acidic Organelles

This paper introduces a far-red dye that selectively labels acidic organelles in live cells, enabling multicolor, time-lapse, and super-resolution imaging with improved photostability and compatibility with long-term experiments.

Direct, quantitative, and comprehensive analysis of tRNA acylation using intact tRNA liquid-chromatography mass-spectrometry

This paper introduces an LC-MS assay to directly and quantitatively analyze tRNA acylation by detecting intact acyl-tRNA products, with sensitivity down to 0.23%.

Aminobenzoic acid derivatives obstruct induced fit in the catalytic center of the ribosome

This study reports cryo-EM structures of E. coli ribosomes with aminobenzoic acid derivatives, revealing how steric blocking and proton wire disruption affect amide bond formation efficiency.

Long-term, super-resolution HIDE imaging of the inner mitochondrial membrane in live cells with a cell-permeant lipid probe

Dose-dependent nuclear delivery and transcriptional repression with a cell-penetrant MeCP2

This study shows that ZF-tMeCP2, a cell-penetrant MeCP2 variant, can deliver to the nucleus and repress transcription from methylated promoters, suggesting a potential therapeutic strategy for Rett syndrome.

Redirecting RiPP biosynthetic enzymes to proteins and backbone-modified substrates

This paper demonstrates that RiPP enzymes can incorporate backbone-modified monomers into proteins, creating sequence-defined chemical polymers with diverse non-alpha-amino acid subunits.

Extremely bright, near-IR emitting spontaneously blinking fluorophores enable ratiometric multicolor nanoscopy in live cells

The paper introduces a new near-IR fluorophore that enables two-color live-cell SMLM without chemical additives or multiple lasers.

Coiled coil control of growth factor and inhibitor-dependent EGFR trafficking anddegradation

The paper identifies a coiled coil switch in the EGFR juxtamembrane segment that controls trafficking and degradation, influencing signaling outcomes and TKI efficacy.

Structure of the Bacterial Ribosome at 2 A Resolution

This paper presents a 2.0 Å resolution model of the E. coli 70S ribosome using cryo-EM, revealing detailed chemical interactions, modifications, and solvation.

Defects in the assembly of ribosomes selected for β-amino acid incorporation

This study investigates a mutant ribosome, P7A7, evolved for β-amino acid incorporation, revealing defects in its assembly that reduce in vitro activity.

Two-color nanoscopy of organelles for extended times with HIDE probes

This paper introduces a new fluorophore and labeling strategy to enable long-term, multi-color nanoscopy of organelles with HIDE probes.

HOPS-dependent endosomal fusion required for efficient cytosolic delivery of therapeutic peptides and small proteins