A. Staes

A multi-year longitudinal harmonization of quality controls in mass spectrometry proteomics core facilities

This study harmonizes quality controls across proteomics core facilities to improve data comparability and reliability through a 4-year longitudinal dataset and common processing software.

High-throughput nano-flow proteomics using a dual-column electrospray source

The paper introduces a dual-column electrospray source for nano-flow proteomics to improve throughput while maintaining sensitivity in low-input samples.

Novel assays monitoring direct glucocorticoid receptor protein activity exhibit high predictive power for ligand activity on endogenous gene targets

The paper introduces novel luciferase-based assays to monitor GR protein activity and shows that GR Ser211 phosphorylation is a reliable predictor of ligand effects on endogenous gene targets.

The use of hybrid data-dependent and -independent acquisition spectral libraries empower dual-proteome profiling

This paper develops a hybrid spectral library approach combining data-dependent and in silico predicted data-independent acquisition proteomics to improve detection of host-pathogen proteomes during bacterial infections.

A simple approach for accurate peptide quantification in MS-based proteomics

This paper introduces a method to accurately quantify MS-ready peptides using a microfluidic UV/visible spectrophotometer, improving LC-MS/MS and protein quantification data consistency.

A well-ordered nanoflow LC-MS/MS approach for proteome profiling using 200 cm long micro pillar array columns

This paper introduces a long micro pillar array column for proteome profiling, showing improved protein identification and peak resolution compared to conventional columns.