S. Eyckerman

Filter-aided extracellular vesicle enrichment (FAEVEr) for proteomics

This paper introduces FAEVEr, a method for enriching extracellular vesicles for proteomics, and shows it effectively isolates EVs from complex samples.

Leveraging a self-cleaving peptide for tailored control in proximity labeling proteomics

The paper introduces a T2A split-link system for proximity labeling proteomics to improve control over enzymatic activity and expression levels, enhancing accuracy in identifying protein interactions.

Activation of goblet cell stress sensor IRE1β is controlled by the mucin chaperone AGR2

This study identifies AGR2 as a chaperone that regulates IRE1β activity in goblet cells by preventing its dimerization and endonuclease activity.

MX2 restricts HIV-1 and herpes simplex virus type 1 by forming cytoplasmic biomolecular condensates that mimic nuclear pore complexes

This study shows MX2 forms cytoplasmic condensates that mimic nuclear pore complexes to restrict HIV-1 and HSV-1 by trapping viral capsids and preventing nuclear entry.

The fork restart factor PHF6 interacts with RRM2 and binds to H3K56ac marked nascent DNA

This study identifies PHF6's interaction with RRM2 and its binding to H3K56ac marked nascent DNA, revealing a role in replication fork restart and DNA damage repair.

Novel assays monitoring direct glucocorticoid receptor protein activity exhibit high predictive power for ligand activity on endogenous gene targets

The paper introduces novel luciferase-based assays to monitor GR protein activity and shows that GR Ser211 phosphorylation is a reliable predictor of ligand effects on endogenous gene targets.

mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cells

This study shows that mTOR inhibition improves the delivery and effectiveness of antisense oligonucleotides in uveal melanoma cells and other cell types.

Ring Finger Protein 213 Assembles into a Sensor for ISGylated Proteins with Antimicrobial Activity

This study identifies RNF213 as an ISG15-interacting sensor that detects ISGylated proteins and exhibits broad antimicrobial activity against multiple pathogens.

The long non-coding RNA SAMMSON is essential for uveal melanoma cell survival

This study shows that inhibiting the lncRNA SAMMSON reduces uveal melanoma cell growth and viability by impairing mitochondrial function and protein translation.

RRM2 is a target for synthetic lethal interactions with replication stress checkpoint addiction in high-risk neuroblastoma

This study identifies RRM2 as a therapeutic target in high-risk neuroblastoma, showing that combined RRM2 and CHK1 inhibition synergistically reduces tumor growth.

IRE1β negatively regulates IRE1α signaling in response to endoplasmic reticulum stress

This study shows IRE1β suppresses IRE1α signaling during ER stress by blocking its activation and disrupting a feedback loop that regulates IRE1 expression.

A well-controlled BioID design for endogenous bait proteins

The paper introduces a controlled BioID design for endogenous p53 using CRISPR/Cas9 and base editors, showing improved proteomic results over ectopic expression.