D. De Sutter

Leveraging a self-cleaving peptide for tailored control in proximity labeling proteomics

The paper introduces a T2A split-link system for proximity labeling proteomics to improve control over enzymatic activity and expression levels, enhancing accuracy in identifying protein interactions.

Activation of goblet cell stress sensor IRE1β is controlled by the mucin chaperone AGR2

This study identifies AGR2 as a chaperone that regulates IRE1β activity in goblet cells by preventing its dimerization and endonuclease activity.

MX2 restricts HIV-1 and herpes simplex virus type 1 by forming cytoplasmic biomolecular condensates that mimic nuclear pore complexes

This study shows MX2 forms cytoplasmic condensates that mimic nuclear pore complexes to restrict HIV-1 and HSV-1 by trapping viral capsids and preventing nuclear entry.

Novel assays monitoring direct glucocorticoid receptor protein activity exhibit high predictive power for ligand activity on endogenous gene targets

The paper introduces novel luciferase-based assays to monitor GR protein activity and shows that GR Ser211 phosphorylation is a reliable predictor of ligand effects on endogenous gene targets.

Ring Finger Protein 213 Assembles into a Sensor for ISGylated Proteins with Antimicrobial Activity

This study identifies RNF213 as an ISG15-interacting sensor that detects ISGylated proteins and exhibits broad antimicrobial activity against multiple pathogens.

IRE1β negatively regulates IRE1α signaling in response to endoplasmic reticulum stress

This study shows IRE1β suppresses IRE1α signaling during ER stress by blocking its activation and disrupting a feedback loop that regulates IRE1 expression.

A well-controlled BioID design for endogenous bait proteins

The paper introduces a controlled BioID design for endogenous p53 using CRISPR/Cas9 and base editors, showing improved proteomic results over ectopic expression.