F. Impens

A multi-year longitudinal harmonization of quality controls in mass spectrometry proteomics core facilities

This study harmonizes quality controls across proteomics core facilities to improve data comparability and reliability through a 4-year longitudinal dataset and common processing software.

High-throughput nano-flow proteomics using a dual-column electrospray source

The paper introduces a dual-column electrospray source for nano-flow proteomics to improve throughput while maintaining sensitivity in low-input samples.

Integrating multiple omics levels using the human protein complexome as a framework, a multi-omics study of inborn errors of metabolism

This study integrates proteomics, transcriptomics, and metabolomics data from IEM patients to identify perturbed protein complexes and pathways, highlighting mitochondrial oxidative phosphorylation and histone acetylation dysregulation.

Aberrant phase separation is a common killing strategy of positively charged peptides in biology and human disease

The paper identifies a common toxic mechanism in positively charged peptides across species and develops a machine learning tool to detect it.

Immunopeptidomics-based design of highly effective mRNA vaccine formulations against Listeria monocytogenes

The paper identifies Listeria monocytogenes epitopes using immunopeptidomics and shows that mRNA vaccines encoding these antigens induce strong immune responses and protection in mice.

Single-cell profiling identifies a spectrum of human unconventional intraepithelial T lineage cells

The paper identifies a spectrum of human unconventional intraepithelial T lineage cells using single-cell RNA sequencing and defines their phenotypic and transcriptomic features.

Pneumococcus uses COMMD2 to alter host cellular immunity.

This study identifies COMMD2 as a bacterial factor that interacts with p65 and p62 to degrade p65, altering host cellular immunity during pneumococcal infection.

Proteome-wide cellular thermal shift assay reveals novel crosstalk between brassinosteroid and auxin signaling

The paper uses CETSA MS to identify novel crosstalk between brassinosteroid and auxin signaling in Arabidopsis by analyzing the thermal and phospho-proteomes of the GSK3 inhibitor bikinin.

Optineurin links Hace1-dependent Rac ubiquitylation to integrin-mediated mechanotransduction to control bacterial invasion and cell division

This study shows OPTN regulates bacterial invasion by linking HACE1-mediated Rac1 ubiquitylation to integrin mechanotransduction and ECM stiffness.

Ring Finger Protein 213 Assembles into a Sensor for ISGylated Proteins with Antimicrobial Activity

This study identifies RNF213 as an ISG15-interacting sensor that detects ISGylated proteins and exhibits broad antimicrobial activity against multiple pathogens.

Establishment of Proximity-dependent Biotinylation Approaches in Different Plant Model Systems

The paper establishes TurboID as the most effective PDL method in plant systems and demonstrates its utility in capturing protein interactomes and structural information.

A simple approach for accurate peptide quantification in MS-based proteomics

This paper introduces a method to accurately quantify MS-ready peptides using a microfluidic UV/visible spectrophotometer, improving LC-MS/MS and protein quantification data consistency.

A well-ordered nanoflow LC-MS/MS approach for proteome profiling using 200 cm long micro pillar array columns

This paper introduces a long micro pillar array column for proteome profiling, showing improved protein identification and peak resolution compared to conventional columns.

Crippling the bacterial cell wall molecular machinery

This study identifies the role of lytic transglycosylase LtgA in bacterial cell wall integrity and shows that targeting it can impair Neisseria meningitidis fitness during infection.