B. Poolman

Ergosterol mediates aggregation of natamycin in the yeast plasma membrane

This paper shows that ergosterol mediates natamycin aggregation in yeast plasma membranes, which underlies its antifungal activity and can be enhanced by sphingolipid inhibitors.

Fitness landscape of substrate-adaptive mutations in evolved APC transporters

This study shows how mutations in yeast APC transporters expand substrate range while affecting fitness for original substrates, revealing trade-offs in transporter evolution.

Probing the stability and interdomain interactions in the ABC transporter OpuA, using single-molecule optical tweezers

The paper uses single-molecule optical tweezers to study the dynamics and interdomain interactions of the ABC transporter OpuA in lipid nanodiscs.

Simulation-based Reconstructed Diffusion unveils the effect of aging on protein diffusion in Escherichia coli

The paper develops SbRD to correct diffusion coefficients in E. coli and finds slower diffusion at cell poles compared to the nucleoid, independent of polysomes, with asymmetry due to aging.

Membrane thickness, lipid phase and sterol type are determining factors in the permeability of membranes to small solutes

The paper investigates how membrane thickness, lipid phase, and sterol type affect permeability to small solutes using synthetic membranes and simulations, finding that ordered states significantly reduce permeability.

Stability of ligand-induced protein conformation influences affinity in maltose-binding protein

The paper shows that altering the stability of ligand-bound conformations can change ligand affinity in maltose-binding protein, beyond modifying binding site residues.

Structural and biophysical characterization of the tandem substrate-binding domains of the ABC importer GlnPQ

This paper characterizes the structural and biophysical properties of the tandem substrate-binding domains of the ABC importer GlnPQ using crystal structures, calorimetry, and smFRET.

Oxygen-independent chemogenetic protein tags for live-cell fluorescence microscopy

This paper introduces oxygen-independent chemogenetic protein tags that enhance fluorescence and enable prolonged live-cell imaging under oxygen-free conditions.

Periprotein lipidomes of Saccharomyces cerevisiae provide a flexible environment for conformational changes of membrane proteins

The paper investigates periprotein lipidomes in yeast membrane microdomains, finding localized lipid differences that suggest a disordered environment for protein function.

Membrane lipid requirements of the lysine transporter Lyp1 from Saccharomyces cerevisiae

The paper identifies essential membrane lipids for the function of the yeast lysine transporter Lyp1 and proposes a model for lipid-protein interactions.

Periprotein membrane lipidomics and the role of lipids in transporter function in yeast

The paper investigates lipid composition in yeast plasma membrane microdomains and identifies lipid requirements for transporter function.

Conformational and dynamical plasticity in substrate-binding proteins underlies selective transport in ABC importers

This study investigates how substrate-binding proteins (SBPs) in ABC importers change conformation and dynamics in response to different ligands, revealing that transport specificity arises from conformational plasticity rather than a unique closed conformation.