C. F. Kaminski

The paper investigates how fusogenic liposomes interact with Gram-negative and Gram-positive bacterial envelopes using microscopy, revealing differences in fusion and delivery mechanisms.

This study demonstrates the use of supported lipid bilayers derived from outer membrane vesicles to monitor specific bacteriophage interactions with bacterial membranes.

This study investigates how mutant FUS protein accumulation affects cytoskeletal, organelle, and cellular homeostasis, linking it to cellular aging and potential senescence mechanisms.

The paper improves the center-of-mass method for fluorescence lifetime imaging by enhancing photon efficiency and robustness to background noise across large dynamic ranges.

ERnet quantifies ER topology dynamics using video-rate super-resolution imaging and semantic segmentation, enabling analysis of ER morphology changes in response to genetic or metabolic perturbations.

This paper shows that Aβ42 aggregation in live cells increases intracellular temperature, which can be mitigated by aggregation inhibitors and is independent of mitochondrial damage.

This paper introduces a method called nano-cavity diffusional sizing (NDS) to measure the size of nanoscale particles and single biomolecules using residence times in nano-cavities.

This paper introduces nano-fluidic traps that increase detection times for single macromolecules and colloids in solution by providing local steric confinement.

The paper uses correlative AFM and SIM to characterize E. coli-derived supported lipid bilayers at the nanoscale, revealing their composition and interactions with membrane-binding proteins.

This study uses cryo-soft-X-ray tomography to image HSV-1 infected cells in near-native state, revealing morphological changes in organelles like mitochondria and cytoplasmic vesicles.

This paper introduces a computational method to analyze high-throughput super-resolution single-particle trajectories, enabling reconstruction of organelle dynamics and membrane reorganization.

This study tracks SARS-CoV-2 structural proteins' spatiotemporal dynamics during infection, revealing nucleocapsid's early association with ER membranes and lysosome involvement in viral egress.

This study identifies distinct domain requirements of EAP45 in HIV budding, late endosomal recruitment, and cytokinesis using fluorescent labeling and microscopy.

The paper creates and tests chimeric BRC repeats to improve RAD51 binding, finding that shuffled modules can enhance binding affinity and function in human cells.

This paper develops a method to measure chromatin compaction in live and fixed cells using SiR-DNA fluorescence lifetime, revealing compaction in differentiating cells and decompaction during stem cell transitions.

This study investigates how Pseudomonas aeruginosa adapts its central carbon metabolism to different carbon sources, revealing distinct metabolic flux patterns and gene expression changes.

This paper combines sample expansion with light sheet microscopy to achieve high-resolution 3D imaging of virus-infected cells, revealing new details of the infection cycle.

This study shows lysosomes actively regulate ER remodeling by repositioning, affecting ER tubule distribution and morphology in response to metabolic changes.

This study uses a recombinant reporter virus to analyze the spatiotemporal dynamics of host cell modifications during herpes simplex virus 1 replication.

This study uses DNA origami nanostructures functionalized with aptamers to deliver lysozyme specifically to bacteria, showing improved efficacy against Gram-positive and Gram-negative targets.

OptiJ is an open-source OPT system for 3D imaging of large transparent samples, offering low-cost, modular hardware and software with improved reconstruction capabilities.

This study uses fast TG-FLIM to analyze α-synuclein and polyglutamine aggregation in aging C. elegans, revealing differences in their aggregation kinetics and amyloid-like features.

This study shows that age-dependent protein aggregation in C. elegans forms amyloid-like structures and accelerates tissue functional decline, suggesting a role for protein aggregation in aging.

The paper combines structured illumination microscopy with machine learning to analyze and classify virus structures efficiently.

The paper develops TriPer, an optical probe that detects H2O2 in the ER, revealing glutathione's role in H2O2 turnover during protein folding.