D. P. Shepherd

Approaching Maximum Resolution in Structured Illumination Microscopy via Accurate Noise Modeling

This paper introduces B-SIM, an unsupervised Bayesian framework for structured illumination microscopy that improves resolution and contrast by accurately modeling noise, outperforming existing methods in both simulated and experimental data.

Projective oblique plane structured illumination microscopy

This paper introduces OPSIM, a structured illumination microscopy technique that enables rapid whole-cell imaging with doubled resolution, achieving up to 2.7 Hz for mitochondrial and ER dynamics.

Resolution doubling in light-sheet microscopy via oblique plane structured illumination

This paper demonstrates isotropic resolution doubling in light-sheet microscopy using oblique plane structured illumination, achieving sub-150nm lateral resolution with reduced phototoxicity and faster volumetric imaging.

Inferring gene expression models from snapshot RNA data

The paper introduces a Bayesian nonparametric method to infer gene networks from single-cell RNA data by simultaneously learning gene states, connectivities, and rate parameters.

A computational proposal for tracking multiple molecules in a multi-focus confocal setup

This paper proposes a computational method to track multiple fluorescent molecules in a multi-focus confocal setup, enabling 3D high-speed tracking below the diffraction limit.

Miniature Structured Illumination Microscope for in vivo 3D Imaging of Brain Structures with Optical Sectioning

The paper introduces a miniature fluorescence microscope for high-resolution 3D brain imaging with optical sectioning, demonstrating its use in fixed and living mouse brain tissue.

Multicolor structured illumination microscopy and quantitative control of polychromatic coherent light with a digital micromirror device

This paper develops a method to enable multicolor coherent structured illumination microscopy using a digital micromirror device, overcoming the blazed grating effect and demonstrating improved resolution and signal-to-noise.

A Versatile Oblique Plane Microscope for Large-Scale and High-Resolution Imaging of Subcellular Dynamics.

This paper introduces an improved oblique plane microscope with enhanced resolution and usability, demonstrating its application in imaging various subcellular dynamics and challenging biological phenomena.

High throughput imaging identifies a spatially localized response of primary fetal pulmonary artery endothelial cells to insulin-like growth factor 1 treatment

The paper uses high throughput imaging to show primary fetal pulmonary artery endothelial cells exhibit spatially localized responses to IGF-1 treatment, revealing cell-to-cell variability not captured by population-level assays.